MColiPAT kit for early detection of coliforms in water
MColiPAT kit for early detection of coliforms in water
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MColiPAT 试剂盒用于早期检测水中的大肠菌群
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
AkhilendraBhushan Gupta
中科院分区：
文献类型：
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作者：
Ashish Tambi;U. Brighu;AkhilendraBhushan Gupta
通过评估指示细菌的存在/不存在（P/A）或枚举来确定饮用水的微生物质量，在世界范围内继续被广泛采用。但是，水质微生物评估需要进行快速测试，以便在尽可能短的时间内获得信息，以便及时采取干预措施。传统的指示菌计数方法不仅费用昂贵，而且需要训练有素的人员。我们开发了一种低成本的试剂盒ColiPAT，并使用IDEXX Colilert-18定量托盘法验证了其在饮用水中大肠菌群检测中的应用。ColiPAT试剂盒培养基能够在10.5小时内检测到3.1 MPN/100 ml的大肠菌群。试剂盒的灵敏度为95.45%，特异性为100%。ColiPAT检测饮用水中大肠菌群可靠、准确。Ashish Tambi（通讯作者）Urmila Brighu A. B. Gupta， Malaviya国立理工学院土木工程系，Jaipur, 302017, Rajasthan，印度E-mail: ashishtambi006@gmail.com
Determining the microbial quality of drinking water by assessing the presence/absence (P/A) or enumeration of indicator bacteria continues to be widely practiced worldwide. However, rapid tests are required for microbiological water quality assessment so that the information is available in the shortest possible time for initiating a timely intervention. Traditional methods for the enumeration of indicator bacteria are not only expensive but also need trained personnel. We have developed a low-cost kit, ColiPAT, and have validated its application for detection of coliforms in drinking water using the IDEXX Colilert-18 Quanti tray method. ColiPAT kit medium was able to detect coliforms down to a level of 3.1 MPN/100 ml within 10.5 hours. The sensitivity and specificity of the kit were 95.45% and 100% respectively. ColiPAT is found to be reliable and accurate for the detection of coliforms in drinking water. doi: 10.2166/ws.2020.008 ://iwaponline.com/ws/article-pdf/20/3/871/765351/ws020030871.pdf Ashish Tambi (corresponding author) Urmila Brighu A. B. Gupta Department of Civil Engineering, Malaviya National Institute of Technology, Jaipur, 302017 Rajasthan, India E-mail: ashishtambi006@gmail.com
DOI：
10.1073/pnas.83.8.2496
发表时间：
1986-04-01
影响因子：
11.1
作者：
BERTHOIS, Y;KATZENELLENBOGEN, JA;KATZENELLENBOGEN, BS
通讯作者：
KATZENELLENBOGEN, BS