Alteration in angiogenic and anti-angiogenic forms of vascular endothelial growth factor-A in skeletal muscle of patients with intermittent claudication following exercise training.
Alteration in angiogenic and anti-angiogenic forms of vascular endothelial growth factor-A in skeletal muscle of patients with intermittent claudication following exercise training.
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DOI：
10.1177/1358863x11436334
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发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
Annex BH
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文献类型：
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作者：
Jones WS;Duscha BD;Robbins JL;Duggan NN;Regensteiner JG;Kraus WE;Hiatt WR;Dokun AO;Annex BH
本研究的目的有两个方面：(1)与对照组相比，外周动脉疾病(PAD)患者肌肉中血管生成的血管生成血管内皮生长因子-A(VEGF-A)、反血管生成的VEGF165b或血管内皮生长因子受体1(VEGF-R1)的浓度是否升高；(2)评估运动训练是否与PAD患者肌肉中血管生成因子-A、VEGF165b或血管内皮生长因子-R1的浓度变化有关。基线时，22名PAD受试者和30名对照组受试者接受了腓肠肌活检。12例PAD患者接受有监督的运动训练(SET)，并在训练3周和12周后进行肌肉活检，并取足够的组织用ELISA法测定骨骼肌裂解物中血管内皮生长因子A、血管内皮生长因子165b和血管内皮生长因子R1的浓度。基线时，PAD患者肌肉中血管内皮生长因子-A和血管内皮生长因子165b的浓度与对照组相似。在SET开始后的两个时间点，VEGF-A水平下降，VEGF165b水平有升高的趋势。在基线时，与对照组相比，PAD患者的VEGF-R1浓度较低，但在SET后没有变化。与基线水平的对照组相比，PAD患者骨骼肌中的血管内皮生长因子-A浓度没有差异。SET与VEGF-A水平显著降低和VEGF165b水平升高的趋势有关。这些有些出乎意料的发现表明，有必要进一步研究PAD患者对运动训练的血管反应机制。
The aims of this study were twofold: (1) to identify whether peripheral artery disease (PAD) patients had increased muscle concentration of angiogenic VEGF-A, anti-angiogenic VEGF165b or VEGF receptor 1 (VEGF-R1) when compared with control subjects, and (2) to evaluate whether exercise training in PAD patients was associated with changes in muscle concentration of VEGF-A, VEGF165b or VEGF-R1. At baseline, 22 PAD and 30 control subjects underwent gastrocnemius muscle biopsy. Twelve PAD patients were treated with supervised exercise training (SET) and underwent muscle biopsy after 3 weeks and 12 weeks of training and had sufficient tissue to measure VEGF-A, VEGF165b and VEGF-R1 concentrations in skeletal muscle lysates by ELISA. Muscle concentrations of VEGF-A and VEGF165b were similar in PAD patients versus controls at baseline. At both time points after the start of SET, VEGF-A levels decreased and there was a trend towards increased VEGF165b concentrations. At baseline, VEGF-R1 concentrations were lower in PAD patients when compared with controls but did not change after SET. Skeletal muscle concentrations of VEGF-A are not different in PAD patients when compared with controls at baseline. SET is associated with a significant reduction in VEGF-A levels and a trend towards increased VEGF165b levels. These somewhat unexpected findings suggest that further investigation into the mechanism of vascular responses to exercise training in PAD patients is warranted.