Molecular diversity and evolution of cystine knot toxins of the tarantula Chilobrachys jingzhao
Molecular diversity and evolution of cystine knot toxins of the tarantula Chilobrachys jingzhao
复制标题
狼蛛Chilobrachys jingzhao胱氨酸结毒素的分子多样性和进化
DOI：
10.1007/s00018-008-8135-x
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发表时间：
2008-08-01
影响因子：
8
通讯作者：
Liang, S.
中科院分区：
文献类型：
--
作者：
Chen, J.;Deng, M.;Liang, S.
蜘蛛毒液中的胱氨酸结毒素（CKTs）是多种离子通道的新型配体的丰富来源。在这里，我们确定了95个新的推定CKT前体通过分析表达序列标签的狼蛛Chilobrachys京兆毒腺。系统发育分析显示，一个孤儿家庭和六个家庭的序列相似性，以已知的毒素。为了进一步研究它们的结构、功能和进化之间的关系，我们测定了10种代表性毒素对离子通道的作用，并进行了结构模型比较、进化分析和毒素分布分析。这项研究揭示了两种主要类型的CKTs：孔阻塞毒素和门控修饰毒素。少数阻断剂具有较高的丰度和广泛的分布，这可能是一类具有较高特异性的阻断多种猎物中保守通道的原始毒素。门控修饰剂家族包含高级毒素，通常具有许多成员并与通道的不同调节组分相互作用。
Cystine knot toxins (CKTs) in spider venoms represent a rich source of novel ligands for varied ion channels. Here, we identified 95 novel putative CKT precursors by analyzing expressed sequence tags of the tarantula Chilobrachys jingzhao venom gland. Phylogenetics analyses revealed one orphan family and six families with sequence similarity to known toxins. To further investigate the relationships of their structures, functions and evolution, we assayed 10 representative toxins for their effect on ion channels, and performed structure model comparisons, evolution analysis and toxin distribution analysis. This study revealed two major types of CKTs: pore-blocking toxins and gating modifier toxins. A few blockers were observed with relatively high abundance and wide distribution, which may be a category of original toxins that block channels conserved in various preys with relatively high specificity. The gating modifier families contain advanced toxins, usually have many members and interact with diverse regulatory components of channels.