Epitope analysis of the cerebrospinal fluid IgG in HTLV-I associated myelopathy patients using phage display method
Epitope analysis of the cerebrospinal fluid IgG in HTLV-I associated myelopathy patients using phage display method
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DOI：
10.1016/j.jneuroim.2004.03.006
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发表时间：
2004-07-01
影响因子：
3.3
通讯作者：
Itoyama, Y
中科院分区：
文献类型：
--
作者：
Fujimori, J;Nakashima, I;Itoyama, Y
我们首次利用显示12个随机肽的噬菌体文库分析了人类嗜T淋巴病毒I型（HTLV-I）相关脊髓病/热带痉挛性麻痹（HAM/TSP）患者脑脊液（CSF）中免疫球蛋白G （IgG）的结合基序。结果表明，与HTLV-I高度同源的gp46 192-199、237-243和255-261序列是患者脑脊液和血清中特异性检测到的高亲和力igg的共同线性表位。这些IgG反应与相应的HTLV-I肽证实，血清抗体滴度与疾病严重程度或持续时间显著相关。Gp46 237-243在之前的酶联免疫吸附试验（ELISA）中未被检测到，这表明噬菌体展示方法是有用的。(C) 2004 Elsevier B.V.版权所有
We, for the first time, analyzed the binding motifs of immunoglobulin G (IgG) in the cerebrospinal fluid (CSF) of human T lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients with a phage library displaying 12-mer random peptides. As a result, the sequences highly homologous to HTLV-I gp46 192-199, 237-243 and 255-261 were the common linear epitopes of high affinity-IgG exclusively detected in both CSF and sera of the patients. These IgG responses were confirmed with corresponding HTLV-I peptides and serum antibody titers significantly correlated with disease severity or duration. Gp46 237-243 has not been detected in previous enzyme-linked immunosorbent assay (ELISA) studies using bound longer peptides, suggesting the usefulness of the phage display method. (C) 2004 Elsevier B.V. All rights reserved.