K-ras gene mutation in gall bladder carcinomas and dysplasia
K-ras gene mutation in gall bladder carcinomas and dysplasia
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DOI：
10.1136/gut.38.3.426
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发表时间：
1996-03-01
期刊：
影响因子：
24.5
通讯作者：
Saitoh, Y
中科院分区：
文献类型：
--
作者：
Ajiki, T;Fujimori, T;Saitoh, Y
胆囊上皮异常增生是胆囊癌发生的重要癌前病变。为探讨胆囊癌和胆囊不典型增生中K-ras基因突变的频率，采用聚合酶链反应/限制性内切酶直接测序法检测胆囊癌和胆囊不典型增生中K-ras基因第12密码子的突变。在59%（30/51）的胆囊癌中检测到突变，在73%（8/11）的胆囊结石病例中的胆囊不典型增生中检测到突变，在0%的正常胆囊上皮中检测到突变。K-ras基因突变与胆囊癌的临床病理因素无关。K-ras基因突变发生，甚至在胆囊发育不良的发病率类似的癌，这表明K-ras基因突变的检测可能被证明是有用的胆汁细胞学或活检分析的辅助。
Epithelial dysplasia of gall bladder is an important precancerous lesion of gall bladder carcinogenesis. To investigate the frequency of K-ras gene mutation in gall bladder carcinoma and dysplasia, K-ras codon 12 mutations were investigated by the polymerase chain reaction/restriction enzyme based method following direct sequencing. Mutation was detected in 59% (30 of 51) of gall bladder carcinomas, in 73% (8 of 11) of gall bladder dysplasia in gall stone cases, and in 0% of the normal gall bladder epithelium. There was, however, no correlation between K-ras mutation and clinicopathological factors of gall bladder carcinoma. K-ras gene mutation occurs even in gall bladder dysplasia at an incidence similar to that in carcinomas, suggesting that testing for K-ras gene mutation may prove useful as an adjunct to bile cytological or biopsy analysis.