Complex interference in the eye developmental pathway by Drosophila NF-YA
Complex interference in the eye developmental pathway by Drosophila NF-YA
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DOI：
10.1002/dvg.20260
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发表时间：
2007-01-01
期刊：
影响因子：
1.5
通讯作者：
Yamaguchi, Masamitsu
中科院分区：
文献类型：
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作者：
Yoshioka, Yasuhide;Suyari, Osamu;Yamaguchi, Masamitsu
CCAAT基序结合因子NF-Y由三个不同的亚基组成，NF-YA，NF-YB和NFYC，所有这些都是形成NF-Y复合物和DNA结合所必需的。NF-YA在其C-末端区域含有DNA结合结构域。我们建立了携带UAS-HA-dNF-YA或UAS-dNF-YAIR的转基因果蝇品系，并显示过表达或敲低各种GAL 4驱动程序在不同发育阶段是致命的，这表明dNF-YA参与果蝇发育过程中的各种基因调控途径。dNF-YA与无眼GAL 4的表达主要导致死亡，在pharate-adults中具有无头表型。减少无眼基因剂量增强了dNF-YA诱导的表型，而减少Distal-less基因剂量抑制了表型。另一方面，将过表达dNF-YA的果蝇与Notch突变体杂交对表型没有明显影响。这些结果表明，dNF-YA可以干扰视盘特化，但不干扰视盘生长。
The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB, and NFYC, all of which are required for formation of the NF-Y complex and DNA-binding. NF-YA contains a DNA binding domain in its C-terminal region. We established transgenic fly lines carrying the UAS-HA-dNF-YA or UAS-dNF-YAIR and showed over-expression or knockdown with various GAL4 drivers to be lethal at various developmental stages, suggesting that dNF-YA participate in various gene regulatory pathways during Drosophila development. Expression of dNF-YA with eyeless-GAL4 mainly resulted in lethality with a headless phenotype in pharate-adults. Reduction of the eyeless gene dose enhanced the dNF-YA-induced phenotype, while reduction of the Distal-less gene dose suppressed the phenotype. On the other hand, crossing the dNF-YA over-expressing flies with Notch mutant resulted in no apparent effect on the phenotype. These results suggest that dNF-YA can disturb eye disc specification, but not eye disc growth.