Proteome analysis of Porphyromonas gingivalis cells placed in a subcutaneous chamber of mice
Proteome analysis of Porphyromonas gingivalis cells placed in a subcutaneous chamber of mice
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DOI：
10.1111/j.1399-302x.2008.00444.x
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发表时间：
2008-10-01
影响因子：
--
通讯作者：
Nakayama, K.
中科院分区：
文献类型：
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作者：
Yoshimura, M.;Ohara, N.;Nakayama, K.
简介：牙龈卟啉单胞菌是一种口腔厌氧细菌，被认为是慢性牙周炎的主要病原体。致病菌通常通过上调或下调基因表达来对抗宿主的保护反应。方法：利用二维凝胶电泳和质谱分析了放置于小鼠皮下腔室的牙龈卟啉卟啉细胞的蛋白质组，以确定当牙龈卟啉卟啉细胞侵入宿主组织时，哪些蛋白受到调控。结果：14个蛋白表达上调，4个蛋白表达下调。我们重点研究了三个上调蛋白，PG1089 (dna结合反应调节因子RprY), PG1385 （TPR结构域蛋白）和PG2102（免疫反应性61-kDa抗原），并构建了这些蛋白缺陷的突变株。小鼠脓肿模型实验表明，PG1385缺陷突变株的毒力明显低于野生型亲本菌株。结论：PG1385蛋白参与了牙龈卟啉卟啉毒力的表达，为进一步研究牙龈卟啉卟啉毒力的表达提供了参考。
Introduction: Porphyromonas gingivalis, an oral anaerobic bacterium, is considered a major pathogen for chronic periodontitis. Pathogenic bacteria usually upregulate or downregulate gene expression to combat the protective responses of their hosts.Methods: To determine what protein is regulated when P. gingivalis cells invade host tissues, we analyzed the proteome of P. gingivalis cells that were placed in a mouse subcutaneous chamber by two-dimensional gel electrophoresis and mass spectrometry.Results: Fourteen proteins were upregulated, while four proteins were downregulated. We focused on three upregulated proteins, PG1089 (DNA-binding response regulator RprY), PG1385 (TPR domain protein), and PG2102 (immunoreactive 61-kDa antigen), and constructed mutant strains that were defective in these proteins. Mouse abscess model experiments revealed that the mutant strain defective in PG1385 was clearly less virulent than the wild-type parent strain.Conclusion: These results indicate that the PG1385 protein is involved in P. gingivalis virulence and that the method used here is useful when investigating the P. gingivalis proteins responsible for virulence.