Retinol-binding protein gene is highly expressed in higher-order association areas of the primate neocortex
Retinol-binding protein gene is highly expressed in higher-order association areas of the primate neocortex
复制标题
DOI：
10.1093/cercor/bhh112
复制
发表时间：
2005-01-01
期刊：
影响因子：
3.7
通讯作者：
Yamamori, T
中科院分区：
文献类型：
--
作者：
Komatsu, Y;Watakabe, A;Yamamori, T
新锥体由组织化学、连接和功能上可区分的区域组成。最近，分子生物学技术使我们能够找到在特定新皮质区域表达的罕见基因类型。我们之前使用差异显示方法报道了 occ1 基因优先在初级视觉皮层（V1）中表达。在这里，通过差异显示，我们发现血清视黄醇结合蛋白（RBP）基因在高阶关联区域中选择性强表达。在V1中，RBP mRNA仅在II层的表层部分表达，但其表达增加，沿着视觉通路涉及更深的层。在 TE 等视觉关联区域，RBP mRNA 在颗粒上层和颗粒下层均强烈表达。在初级听觉和体感区域，如 V1，RBP 表达较低，并且仅限于颗粒上层的上部。 RBP表达的层状模式与occ1的层状模式形成鲜明对比；在这两种基因都表达的早期视觉区域，这些基因出现在颗粒上层内的不同亚层中。在新生猴中，RBP的区域特异性表达模式不太明显，表明RBP在高阶关联区域的特征性表达主要是在出生后建立的。
The neoconex consists of histochemically, connectionally, and functionally distinguishable areas. Recently, molecular biological techniques have enabled us to find rare types of genes expressed in specific neocortical areas. We previously reported occ1 gene as preferentially expressed in the primary visual cortex (V1), using the differential display method. Here, by differential display, we found selective and strong expression of the serum retinol-binding protein (RBP) gene, in higher-order association areas. In V1, RBP mRNA was expressed only in the superficial part of layer II, but its expression increased, involving deeper layers, along the visual pathway. In visual association areas such as TE, RBP mRNA was strongly expressed in both supra- and infragranular layers. In primary auditory and somatosensory areas, as in V1, RBP expression was low, and restricted to the upper part of the supragranular layers. The laminar pattern of RBP expression is in marked contrast with that of occ1; and in early visual areas where both genes are expressed, these occur in distinct sublayers within the supragranular layers. In neonatal monkeys, the area-specific expression pattern of RBP was less distinct, suggesting that the characteristic expression of RBP in higher-order association areas is mainly established postnatally.