Developmentally related and drought-induced shifts in the kale metabolome limited Salmonella enterica association, providing novel insights to enhance food safety
Developmentally related and drought-induced shifts in the kale metabolome limited Salmonella enterica association, providing novel insights to enhance food safety
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羽衣甘蓝代谢组与发育相关和干旱引起的变化限制了肠道沙门氏菌的关联，为增强食品安全提供了新的见解
DOI：
10.1016/j.fm.2022.104113
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发表时间：
2022
影响因子：
5.3
通讯作者：
Micallef, Shirley A.
中科院分区：
文献类型：
--
作者：
Liu, Xingchen;Li, Yue;Micallef, Shirley A.
植物影响附生细菌的关联，但肠道沙门氏菌共生定植在作物植物上，增加了人类食源性疾病的可能性，但介导人类病原体-植物关联的因素仍未得到充分研究。我们评估了羽衣甘蓝（Brassica oleraceaAcephala group）发育过程中叶片组织和表面代谢组是否有任何变化，以及干旱对沙门氏菌叶片关联的响应。羽衣甘蓝叶片组织提取物和叶片表面洗涤的非靶向植物化学分析（包括初级和次级代谢物）揭示了随着植物发育而变化的不同代谢物谱。在干旱胁迫下，羽衣甘蓝幼体的代谢组也出现了分化，而成熟羽衣甘蓝没有发现这一现象。幼株限制浇水导致45种化合物在叶片组织中积累，21种化合物在叶片洗涤液中积累，并出现几个独特的峰，同时植物化学测量值增加。成熟植物叶片提取物的抗氧化能力和总黄酮含量高于定期浇水的幼叶提取物。干旱还会诱导幼嫩植株产生黄酮类化合物和硫代葡萄糖苷。在成熟植物中，干旱没有引起进一步的显著变化。经常浇水的羽衣甘蓝幼苗为接种的沙门氏菌提供了良好的基质，但其支持沙门氏菌的能力随着年龄和干旱胁迫而下降。与对照相比，在成熟或缺水的植物洗涤中，沙门氏菌的生长受到损害，并且在叶片上和叶片洗涤中检测到沙门氏菌数量之间的正相关。此外，沙门氏菌的数量与羽衣甘蓝幼苗和定期浇水的羽衣甘蓝组织中的总黄酮和酚类物质呈负相关。未来的研究应该评估羽衣甘蓝植株表面初级和次级代谢物的变化如何调节沙门氏菌的关联。在受控制的农业中，有管制的限水可以成为一种战略，具有提高有益健康的质量和食品安全的双重目的，特别是在羽衣甘蓝幼苗阶段收获时。
Plants influence epiphytic bacterial associations but Salmonella enterica colonizes crop plants commensally, raising the possibility of human foodborne illness, but the factors that mediate human pathogen-plant associations remain understudied. We evaluated whether any changes in leaf tissue and surface metabolomes with kale (Brassica oleraceaAcephala group) development and in response to drought modulatedSalmonellaleaf association. Untargeted phytochemical profiling (including primary and secondary metabolites) of kale leaf tissue extracts and leaf surface washes revealed distinct metabolite profiles that shifted with plant development. Metabolomes of juvenile plants also diverged in response to drought stress, an effect not noted in mature kale. Restricted watering in juvenile plants led to up-accumulation of 45 compounds in leaf tissue and 21 in leaf wash and the appearance of several unique peaks, with concomitant increases in phytochemical measurements. The antioxidant capacity and total flavonoid content were higher in mature than juvenile, regularly watered plant leaf extracts. Drought also elicited flavonoids and glucosinolates in juvenile plants. In mature plants, drought did not induce further prominent changes. Regularly watered juvenile kale provided a favorable substrate for inoculatedSalmonellabut the ability to supportSalmonelladeclined with age and with drought stress.Salmonellagrowth was impaired in mature or water-stressed plant washes compared to controls and positive correlations were detected betweenSalmonellacounts on leaves and in leaf washes. Moreover,Salmonellacounts were inversely correlated with total flavonoids and phenolics in kale tissues from juvenile plants and regularly watered plants. Future studies should assess how changes in primary and secondary metabolites on the kale plant surface can modulate theSalmonellaassociation. Regulated water restriction could be a strategy in controlled agriculture, with the dual purpose of enhancing health beneficial quality and food safety, especially when harvested at the baby kale stage.