Characterization and immunolocalization of beta-D-glucuronidase in mouse testicular germ cells and spermatozoa.
Characterization and immunolocalization of beta-D-glucuronidase in mouse testicular germ cells and spermatozoa.
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小鼠睾丸生殖细胞和精子中 β-D-葡萄糖醛酸酶的表征和免疫定位。
DOI：
10.1006/excr.1998.4355
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Tulsiani,DR
中科院分区：
文献类型：
--
作者：
Abou-Haila,A;Fouquet,JP;Tulsiani,DR
从曲细精管释放的精子是终末分化的细胞，没有已知的合成活性。它们的成分在精子发生过程中在生精细胞中合成。在这项研究中，我们报告的特性和β-葡萄糖醛酸酶在小鼠睾丸生殖细胞和精子的免疫定位。该酶是一种具有双重定位的外切糖水解酶，存在于几种小鼠和大鼠组织的溶酶体和内质网中。当测定纯化的生殖细胞制备物（精母细胞、圆形精子细胞和浓缩/伸长精子细胞）的β-葡萄糖醛酸酶活性时，发现精母细胞每个细胞的酶活性是精子细胞的5倍。聚丙烯酰胺凝胶电泳，在天然和变性条件下进行，表明生殖细胞表达的酶（pI5.5-6.0）的溶酶体形式与74 kDa的亚基分子量。免疫细胞化学研究显示，在高尔基体膜，高尔基体相关的囊泡，和溶酶体的晚期精母细胞（粗线期精母细胞）和精子发生过程中的阶段特异性定位的阳性反应。第9-16期精子细胞和附睾精子顶体的形成和形成呈强阳性反应。比较免疫沉淀曲线和动力学性质的酶存在于精母细胞和精子没有发现重大差异。综上所述，我们的结果表明，精母细胞和精子顶体的溶酶体中存在的β-葡萄糖醛酸酶活性在动力学和免疫学上是相似的。
Spermatozoa released from the seminiferous tubules are terminally differentiated cells with no known synthetic activity. Their components are synthesized in the spermatogenic cells during spermatogenesis. In this study, we report the characterization and immunolocalization of β-glucuronidase in mouse testicular germ cells and spermatozoa. The enzyme is an exoglycohydrolase with dual localization, being present in lysosomes and endoplasmic reticulum of several mouse and rat tissues. The purified germ cell preparations (spermatocytes, round spermatids, and condensed/elongated spermatids) when assayed for β-glucuronidase activity showed that the spermatocytes contained five times more enzyme activity per cell than the spermatids. Polyacrylamide gel electrophoresis, carried out under native and denaturing conditions, demonstrated that the germ cells express only the lysosomal form of the enzyme (pI5.5–6.0) with a subunit molecular mass of 74 kDa. Immunocytochemical studies revealed a positive reaction in the Golgi membranes, Golgi-associated vesicles, and lysosomes of late spermatocytes (pachytene spermatocytes) and a stage-specific localization during spermiogenesis. The forming or formed acrosome of the elongated spermatids (stages 9–16) and epididymal spermatozoa was highly immunopositive. Comparison of immunoprecipitation curves and kinetic properties of the enzyme present in spermatocytes and spermatozoa revealed no major differences. Taken together, our results demonstrate that β-glucuronidase activities present in the lysosomes of spermatocytes and the sperm acrosome are kinetically and immunologically similar.
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Mr 46,000 甘露糖 6-磷酸受体的糖基化。
影响因子：
4.8
作者：
M. Wendland;A. Waheed;B. Schmidt;A. Hille;G. Nagel;K. von Figura;R. Pohlmann
通讯作者：
R. Pohlmann
大鼠精子相关α-L-岩藻糖苷酶的合成和加工。
影响因子：
3.6
作者：
Hancock,LW;Raab,LS;AronsonJr,NN
通讯作者：
AronsonJr,NN
DOI：
10.1016/s0079-6603(08)60698-4
发表时间：
1989
期刊：
Progress in nucleic acid research and molecular biology
影响因子：
--
作者：
Kenneth Paigen
通讯作者：
Kenneth Paigen
雌性大鼠包皮腺多种形式β-葡萄糖醛酸酶的制备及其化学成分。
DOI：
10.1016/s0021-9258(19)41369-0
发表时间：
1975
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Preputial;Gland;Ronald K. Keller;O. Touster
通讯作者：
O. Touster
免疫过氧化物酶染色未能检测精子赤道段中的顶体素 (1)。
DOI：
--
发表时间：
1977
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
D. Garner;S. A. Reamer;L. Johnson;B. A. Lessley
通讯作者：
B. A. Lessley