Co-occurrence of microplastics and triclosan inhibited nitrification function and enriched antibiotic resistance genes in nitrifying sludge
Co-occurrence of microplastics and triclosan inhibited nitrification function and enriched antibiotic resistance genes in nitrifying sludge
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DOI：
10.1016/j.jhazmat.2020.123049
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发表时间：
2020-11-15
影响因子：
13.6
通讯作者：
Zhao, Yifan
中科院分区：
文献类型：
--
作者：
Wang, Zhiqi;Gao, Jingfeng;Zhao, Yifan
随着越来越多的消费品中添加的微塑料（MP）和三氯生（TCS）随污水进入污水处理厂，人们担心MP和TCS共存对生物污水处理的影响。在实验室规模的硝化序批式反应器（SBR）中，研究了聚乙烯（PE）、聚苯乙烯（PS）、聚氯乙烯（PVC）和聚酰胺（PA）4种1 mg/L多聚电解质（MP）与0.5 mg/L三氯甲烷（TCS）的协同硝化作用（SBR-PE、SBR-PS、SBR-PVC和SBR-PA），并以未添加MP的对照（SBR-CK）为对照。SBR-CK对NH 4 +-N和TCS的去除率分别在100%和92%左右。与SBR-CK相比，SBR-PE和SBR-PS对硝化作用没有明显的抑制作用，而SBR-PVC和SBR-PA在14 d内迅速丧失硝化作用，这可能是由于PVC、PA和TCS共同负荷导致MLSS降低所致。此外，PS、PVC和PA降低了TCS的去除。TCS与PS、PVC、PA的共存增加了胞外聚合物，降低了微生物多样性，改变了微生物群落。值得注意的是，acrA-03、mexF、fabI、intI 1、intI 3和IS 613基因通过MP和TCS共加载而富集。因此，应优先考虑从废水中去除MP和TCS。
As more and more microplastics (MPs) and triclosan (TCS), which are added in consumer products, enter wastewater treatment plants with sewage, there are concerns about the impacts of the co-occurrence of MPs and TCS on biological wastewater treatment. In this study, the co-effects of four 1 mg/L MPs (polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC) and polyamide (PA)) and 0.5 mg/L TCS on nitrification were investigated in lab-scale nitrifying sequencing batch reactors (SBRs) (SBR-PE, SBR-PS, SBR-PVC and SBR-PA) relative to control which received no MPs (SBR-CK). The removal rates of NH4+-N and TCS in SBR-CK were around 100% and 92%, respectively. Compared with SBR-CK, no measurable inhibition was observed on nitrification in SBR-PE and SBR-PS, however, SBR-PVC and SBR-PA rapidly lost nitrification function during 14 days, which might be due to the reducing of MLSS caused by PVC, PA and TCS co-loading. Furthermore, PS, PVC and PA decreased the removal of TCS. The co-occurrence of TCS and PS, PVC, PA increased extracellular polymeric substances, reduced microbial diversity and shifted microbial communities. Notably, the acrA-03, mexF, fabI, intI1, intI3 and IS613 genes were enriched by MPs and TCS co-loading. Therefore, the removal of MPs and TCS from wastewater should be prioritized.