PROCESSED ANTIGEN BINDS TO NEWLY SYNTHESIZED MHC CLASS II MOLECULES IN ANTIGEN-SPECIFIC LYMPHOCYTES-B
PROCESSED ANTIGEN BINDS TO NEWLY SYNTHESIZED MHC CLASS II MOLECULES IN ANTIGEN-SPECIFIC LYMPHOCYTES-B
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DOI：
10.1016/0092-8674(91)90575-j
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发表时间：
1991-10-04
期刊：
影响因子：
64.5
通讯作者：
WATTS, C
中科院分区：
文献类型：
--
作者：
DAVIDSON, HW;REID, PA;WATTS, C
我们描述了在免疫球蛋白介导的内吞作用和 B 类淋巴母细胞中天然抗原的加工后直接检测与 II 类 MHC 分子结合的放射性标记抗原片段。 Tris-Tricine SDS 凝胶显示出六种不同的碘化加工产物，这些产物可在抗原内吞后 1 小时在 II 类 MHC 上检测到，并持续至少 20 小时。 这些生理加工的抗原 II 类复合物非常稳定，这是通过以下事实来判断的：II 类-α-β 二聚体（仍与 SDS 结合）被同一组加工肽标记。 使用凝集素结合测定，我们表明这些生理加工产物与新成熟的 MHC 分子群体结合，而不是与预先存在的细胞表面群体结合；相反，外源肽主要与后一群体结合。 对加工肽-MHC 复合物形成的直接 T 细胞独立测定应该有助于对外源抗原加工途径的进一步研究。
We describe the direct detection of radiolabeled antigen fragments bound to class II MHC molecules following immunoglobulin-mediated endocytosis and processing of native antigen in B lymphoblastoid cells. Tris-Tricine SDS gels revealed six distinct iodinated processing products that could be detected on class II MHC 1 hr after antigen endocytosis and persisted for at least 20 hr. These physiological processed antigen-class II complexes were remarkably stable, as judged by the fact that class II-alpha-beta dimers, which remain associated in SDS, became labeled with the same set of processed peptides. Using a lectin-binding assay, we show that these physiological processing products bind to the newly maturing population of MHC molecules rather than binding to the preexisting cell surface population; in contrast, an exogenous peptide binds predominantly to the latter population. A direct T cell-independent assay for processed peptide-MHC complex formation should facilitate additional studies on the exogenous antigen processing pathway.