Ordered assembly of mitochondria during rice germination begins with promitochondrial structures rich in components of the protein import apparatus
Ordered assembly of mitochondria during rice germination begins with promitochondrial structures rich in components of the protein import apparatus
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DOI：
10.1007/s11103-005-3688-7
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发表时间：
2006-01-01
影响因子：
5.1
通讯作者：
Whelan, J
中科院分区：
文献类型：
--
作者：
Howell, KA;Millar, AH;Whelan, J
水稻胚吸胀过程中线粒体的成熟是由非结构化的双膜结合的原线粒体向成熟植物细胞中典型的富含嵴的线粒体结构转变。在吸胀后的第一个48小时内，观察到编码线粒体蛋白的转录本丰度的有序增加。与转录水平的这些变化同时发生的是线粒体蛋白含量和线粒体功能的动态和快速变化。线粒体蛋白质进口装置的组成部分的蛋白质是惊人的丰富的干种子，和功能的进口装置被证明是吸胀后2小时操作。有趣的是，这个输入过程最好是由线粒体内膜外的NADH氧化驱动的。在后来的发展阶段，基质有机酸代谢的能力是显而易见的，伴随着TCA循环组件的蛋白质的外观，并通过在线粒体和核基因组中编码的组件的电子传递链组装的协调。这些事件为理解植物细胞中线粒体成熟和原线粒体结构的性质提供了新的见解。
Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound promitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts encoding mitochondrial proteins was observed. Co-incident with these changes in transcript levels was dynamic and rapid changes in mitochondrial protein content and mitochondrial function. Proteins representing components of the mitochondrial protein import apparatus are strikingly abundant in dry seeds, and a functional import apparatus was shown to operate 2 h after imbibition. Interestingly, this import process was best driven by the oxidation of NADH from outside the mitochondrial inner membrane. In later developmental stages the capacity for matrix organic acid metabolism was evident, accompanied by the appearance of proteins for TCA cycle components, and coordination of electron transport chain assembly through components encoded in both mitochondrial and nuclear genomes. Together these events provide new insights into the understanding of mitochondrial maturation and the nature of promitochondrial structures in plant cells.