Capillary electrochromatography and preconcentration of neutral compounds on poly(dimethylsiloxane) microchips
Capillary electrochromatography and preconcentration of neutral compounds on poly(dimethylsiloxane) microchips
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DOI：
10.1002/elps.200305568
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发表时间：
2003-09
期刊：
影响因子：
2.9
通讯作者：
K. Ro;Woo-Jin Chang;Ho Kim;Y. Koo;J. Hahn
中科院分区：
文献类型：
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作者：
K. Ro;Woo-Jin Chang;Ho Kim;Y. Koo;J. Hahn
在聚二甲基硅氧烷（PDMS）微芯片上实现了毛细管电色谱（CEC）和中性化合物的预富集。通道涂有聚电解质多层，以避免疏水性分析物吸收到PDMS中。微芯片的结构包括注射器和具有集成玻璃料的珠室，其中固定相的颗粒被完全保留。熔块结构尺寸为25 μm×20 μm，结构间距为3 μm。中性化合物BODIPY被天然PDMS强烈吸收，成功地选择性地保留在珠室中十八烷基硅烷包被的二氧化硅珠上，浓度提高高达100倍，并用含有70%乙腈的洗脱缓冲液洗脱。用同一装置对香豆素类化合物进行了预浓缩和CEC分离，在50 s内实现了完全分离。
Capillary electrochromatography (CEC) and preconcentration of neutral compounds have been realized on poly(dimethylsiloxane) (PDMS) microchips. The channels are coated with polyelectrolyte multilayers to avoid absorption of hydrophobic analytes into PDMS. The structures of a microchip include an injector and a bead chamber with integrated frits, where the particles of the stationary phase are completely retained. Dimensions of the frit structures are 25 μm×20 μm, and the space between the structures is 3 μm. A neutral compound, BODIPY, that is strongly absorbed into native PDMS, is successfully and selectively retained on octadecylsilane‐coated silica beads in the bead chamber with a concentration enhancement of up to 100 times and eluted with elution buffer solution containing 70% acetonitrile. Preconcentrations and CEC separations of coumarins have been conducted with the same device and achieved complete separations in less than 50 s.