COLLAGEN REDUCES GLYCOSAMINOGLYCAN DEGRADATION BY CULTURED MAMMARY EPITHELIAL-CELLS - POSSIBLE MECHANISM FOR BASAL LAMINA FORMATION
COLLAGEN REDUCES GLYCOSAMINOGLYCAN DEGRADATION BY CULTURED MAMMARY EPITHELIAL-CELLS - POSSIBLE MECHANISM FOR BASAL LAMINA FORMATION
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DOI：
10.1073/pnas.76.2.786
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
BERNFIELD, MR
中科院分区：
文献类型：
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作者：
DAVID, G;BERNFIELD, MR
胶原基质促进培养的上皮细胞细胞外基质物质的积累。在塑料或I型胶原凝胶基质上维持的小鼠乳腺上皮细胞的二次培养物中比较了糖胺聚糖（GAG）代谢。35 SO 42-在短暂标记过程中掺入GAG，表明底物之间的GAG合成速率无差异。在长期标记过程中，胶原蛋白上培养物中[35 S]GAG的积累超过了塑料上培养物的积累。蓄积增加是由于GAG降解速率显著降低所致。培养基中未发生GAG降解，表明降解局限于细胞。胶原蛋白上的培养物含有缓慢降解的细胞相关[35 S]GAG池和Ru红染色的基底层，这两种物质都不存在于塑料上的培养物中。胶原蛋白培养物中的细胞相关[35 S]GAG部分定位于超微结构鉴定的基底层部位。基底层的形成可能是胶原介导的含GAG分子降解减少所致。
Collagenous substrates promote the accumulation of extracellular matrix materials by epithelia in culture. Glycosaminoglycan (GAG) metabolism was compared in secondary cultures of mouse mammary epithelial cells maintained on plastic or type I collagen gel substrates. The incorporation of 35SO42- into GAG during brief labeling indicated no difference between substrates in the rate of GAG synthesis. During prolonged labeling accumulation of [35S]GAG in cultures on collagen exceeded that of cultures on plastic. This increased accumulation was due to a markedly reduced rate of GAG degradation. GAG degradation did not occur in the medium, indicating that degradation was localized to the cells. The cultures on collagen contained slowly degrading cell-associated [35S]GAG pool and a Ru red-stained basal lamina, neither of which is present in cultures on plastic. The cell-associated [35S]GAG in cultures on collagen was partially localized to the site of the ultrastructurally identified basal lamina. Formation of the basal lamina may result from collagen-mediated reduction in the degradation of GAG-containing molecules.