Differences in the expression of microRNAs and their predicted gene targets between cauda epididymal and ejaculated boar sperm
Differences in the expression of microRNAs and their predicted gene targets between cauda epididymal and ejaculated boar sperm
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附睾尾部与射精公猪精子之间 microRNA 表达及其预测基因靶点的差异
DOI：
10.1016/j.theriogenology.2016.07.012
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发表时间：
2016-12-01
期刊：
影响因子：
2.8
通讯作者：
Zeng, Chang-jun
中科院分区：
文献类型：
--
作者：
Chang, Yu;Dai, Ding-hui;Zeng, Chang-jun
哺乳动物精子在从睾丸向附睾头和尾部过渡的过程中逐渐成熟并获得生育力，之后它们被储存在附睾尾部和输精管壶腹。在射精过程中，精子与附属性腺的分泌物混合，导致它们的稀释和功能的改变。虽然对射精前后精子生物化学和功能变化的理解已经取得了显着进展，但在附睾尾和射精之间的过渡期间，microRNAs（miRNAs）是否参与调节精子的功能尚不清楚。在这项研究中，选择了48个miRNAs进行分析的基础上，他们的潜在参与精子发生，精子成熟和质量参数标记。测定了这48个miRNAs在猪精子尾部附睾和新鲜精液中的差异表达水平。我们发现15个miRNAs在猪附睾尾和新鲜精子中有显著差异表达（8个下调和7个上调）。采用定量逆转录聚合酶链反应进一步检测了5种假设参与精子凋亡的miRNAs，以证明其对靶mRNA表达的影响。总之，我们的研究结果表明，这些差异表达的miRNA与附睾尾和射精之间的精子功能调节有关。(C)2016 Elsevier Inc. All rights reserved.
Mammalian spermatozoa gradually mature and acquire fertility during the transition from the testis to the caput and cauda epididymis, after which they are stored at the tail of the epididymis and the ampulla of vas deferens. During ejaculation, mixing of spermatozoa with the secretions of accessory sex glands leads to their dilution and changes in their function. Although remarkable progress has been made toward the understanding of changes in spermatozoa biochemistry and function before and after ejaculation, it is unknown whether microRNAs (miRNAs) are involved in regulating the function of spermatozoa during the transition between the cauda epididymis and ejaculation. In this study, 48 miRNAs were selected for analysis on the basis of their potential involvement in spermatogenesis, sperm maturation, and quality parameters markers. The differential expression levels of these 48 miRNAs between the caudal epididymis and fresh ejaculates of boar spermatozoa were determined. We found that 15 miRNAs were significantly differentially expressed (eight downregulated and seven upregulated) between boar cauda epididymal and fresh spermatozoa. Five miRNAs hypothesized to be involved in sperm apoptosis were further tested to demonstrate their influence over the expression of their target mRNAs using quantitative reverse-transcription polymerase chain reaction. Together, our findings suggest that these differentially expressed miRNAs are associated with the functional regulation of spermatozoa between cauda epididymis and ejaculation. (C) 2016 Elsevier Inc. All rights reserved.