Detailed Reaction Mechanism of Phenylboronic Acid with Alizarin Red S in Aqueous Solution: Re-Investigation with Spectrophotometry and Fluorometry
Detailed Reaction Mechanism of Phenylboronic Acid with Alizarin Red S in Aqueous Solution: Re-Investigation with Spectrophotometry and Fluorometry
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DOI：
10.1002/slct.201700166
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发表时间：
2017-04
期刊：
影响因子：
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通讯作者：
Yota Suzuki;Tomoaki Sugaya;S. Iwatsuki;M. Inamo;H. Takagi;A. Odani;K. Ishihara
中科院分区：
文献类型：
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作者：
Yota Suzuki;Tomoaki Sugaya;S. Iwatsuki;M. Inamo;H. Takagi;A. Odani;K. Ishihara
用吸收和发射光谱法重新研究了苯基硼酸（PhB（OH）2）与茜素红S（ARS，H2 L）的反应，修正了目前提出的反应机理。两种方法得到的动力学结果基本上没有显着差异，表明ARS对PhB（OH）2的动力学反应性顺序为H2 L PhB（OH）3-。乙酸/乙酸盐缓冲液加速本反应，但HEPES缓冲液没有。根据最近提出的一种通用反应机理，提出了一种详细的反应机理，其中包括醋酸盐缓冲液的加速作用。
Re-investigation of the reaction of phenylboronic acid (PhB(OH)2) with Alizarin Red S (ARS, H2L) was carried out using absorption and emission spectrophotometry to correct the currently proposed mechanism. Kinetic results obtained by both methods, which essentially show no significant differences, showed that the kinetic reactivity of ARS toward PhB(OH)2 was in the order H2L PhB(OH)3–. Acetic acid/acetate buffer accelerated the present reaction, though HEPES buffer did not. A detailed reaction mechanism involving the acceleration effect of the acetate buffer is proposed for the present reaction on the basis of a recent universal reaction mechanism.