Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging.
Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging.
复制标题
高度特异性的 C-C 键裂解诱导 FRET 荧光，用于体内生物一氧化氮成像。
DOI：
10.1039/c6sc04071c
复制
发表时间：
2017-03-01
期刊：
影响因子：
8.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Li H;Zhang D;Gao M;Huang L;Tang L;Li Z;Chen X;Zhang X
报道了一种基于某些二氢吡啶类化合物高度特异、灵敏和有效的C-C键断裂的FRET荧光“关-开”系统，用于一氧化氮(NO)的实时定量成像。报道了一种新的Förster共振能量转移(FRET)荧光“OFF-ON”体系，该体系基于某些二氢吡啶类化合物高度特异、灵敏和有效的C-C键断裂，用于一氧化氮(NO)的实时定量成像。合成了1，4-二氢吡啶作为一种新型的连接剂，它可以连接定制的荧光团及其相应的猝灭剂。以异硫氰酸荧光素(FITC)和[4‘-(N，N’-二甲氨基)苯偶氮]苯甲酰基(DABCyL)为例，用荧光光谱学方法证实了NO的特异性和定量响应。随着NO含量的增加，荧光强度呈线性增加。与外源性NO供体孵育的细胞如预期的那样发出荧光。巨噬细胞在与脂多糖(LPS)孵育时产生NO的荧光强度较高。体内成像显示，静脉注射弗氏佐剂刺激的小鼠足与正常足之间的对比度约为8倍，这是首次在哺乳动物体内进行NO的半定量荧光成像。
A novel FRET fluorescence “off–on” system based on the highly specific, sensitive and effective C–C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). A novel Förster resonance energy transfer (FRET) fluorescence “off–on” system based on the highly specific, sensitive and effective C–C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). 1,4-Dihydropyridine was synthesized as a novel linker which could connect customized fluorophores and their corresponding quenchers. The specific and quantitative response to NO is confirmed using fluorescence spectrometry with the classical example of fluorescein isothiocyanate (FITC) and [4′-(N,N′-dimethylamino)phenylazo] benzoyl (DABCYL). The fluorescence intensity increased linearly with the increase in the amount of NO. Cells incubated with an exogenous NO donor emitted fluorescence as expected. A high fluorescence intensity was detected in macrophages which generate NO when incubated with lipopolysaccharide (LPS). The in vivo imaging shows about an 8-fold contrast between Freund's adjuvant stimulated feet and normal feet in mice after intravenous injection, which was the first example of in vivo semiquantitative fluorescence imaging of NO in mammals.