High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa
High expression of ACE2 receptor of 2019-nCoV on the epithelial cells of oral mucosa
复制标题
DOI：
10.1038/s41368-020-0074-x
复制
发表时间：
2020-02-24
影响因子：
14.9
通讯作者：
Chen, Qianming
中科院分区：
文献类型：
--
作者：
Xu, Hao;Zhong, Liang;Chen, Qianming
据报道，ACE2是2019-nCoV的主要宿主细胞受体，在病毒进入细胞引起最终感染的过程中发挥着至关重要的作用。为了研究口腔粘膜上 2019-nCov 感染的潜在途径，收集了来自两个公共数据库的批量 RNA 序列图谱，包括癌症基因组图谱 (TCGA) 和哺乳动物基因组基因表达功能注释 (FANTOM5 CAGE) 数据集。为了探索和验证 ACE2 在口腔粘膜上的表达，分析了来自 TCGA 的 13 个器官类型的癌旁正常组织和来自 FANTOM5 CAGE 的 14 个器官类型的正常组织的 RNA-seq 分析数据。此外，使用内部生成的独立数据的单细胞转录组来识别和确认口腔中表达 ACE2 的细胞组成和比例。结果表明ACE2在口腔粘膜上表达。有趣的是，这种受体在舌上皮细胞中高度富集。这些研究结果初步解释了口腔是2019-nCoV感染易感性潜在高风险的基本机制，为未来牙科临床实践和日常生活中的预防策略提供了证据。
It has been reported that ACE2 is the main host cell receptor of 2019-nCoV and plays a crucial role in the entry of virus into the cell to cause the final infection. To investigate the potential route of 2019-nCov infection on the mucosa of oral cavity, bulk RNA-seq profiles from two public databases including The Cancer Genome Atlas (TCGA) and Functional Annotation of The Mammalian Genome Cap Analysis of Gene Expression (FANTOM5 CAGE) dataset were collected. RNA-seq profiling data of 13 organ types with para-carcinoma normal tissues from TCGA and 14 organ types with normal tissues from FANTOM5 CAGE were analyzed in order to explore and validate the expression of ACE2 on the mucosa of oral cavity. Further, single-cell transcriptomes from an independent data generated in-house were used to identify and confirm the ACE2-expressing cell composition and proportion in oral cavity. The results demonstrated that the ACE2 expressed on the mucosa of oral cavity. Interestingly, this receptor was highly enriched in epithelial cells of tongue. Preliminarily, those findings have explained the basic mechanism that the oral cavity is a potentially high risk for 2019-nCoV infectious susceptibility and provided a piece of evidence for the future prevention strategy in dental clinical practice as well as daily life.