Healing of tympanic membrane after myringotomy during Streptococcus pneumoniae otitis media - An otomicroscopic and histologic study in the rat
Healing of tympanic membrane after myringotomy during Streptococcus pneumoniae otitis media - An otomicroscopic and histologic study in the rat
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DOI：
10.1177/000348949610500513
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发表时间：
1996-05-01
影响因子：
1.4
通讯作者：
Hellstrom, S
中科院分区：
文献类型：
--
作者：
Magnuson, K;Hermansson, A;Hellstrom, S
我们研究的目的是阐明急性中耳炎鼓膜切开术时鼓膜的愈合过程。在动物模型上研究了TM的早期和长期结构变化。大鼠大疱性肺炎链球菌(PNC)3型接种。感染明显时，行鼓膜切开术。在鼓膜切开术后第4天、第12天、第3个月、第6个月，通过耳镜检查TM状态，并制备TM材料进行光镜和电子显微镜检查。与PNC感染的未穿孔的TMS以及鼓膜切开的非感染TMS进行比较。与未接触的PNC感染的耳朵相比，鼓膜切开术的耳朵感染缓解得更慢。6个月后，鼓膜切开后感染耳与TMS正常的鼓膜未感染耳相比，张力部分增厚，胶原结构紊乱。未作鼓膜切开的PNC感染的TMS在2个月后完全正常。我们的结论是，细菌感染和鼓膜切开术的结合会导致TM结构的长期变化。结缔组织的这种受损结构在慢性中耳疾病中可能是重要的，因为它是TM回缩和穿孔的推定部位。
The purpose of our study was to elucidate the course of healing of the tympanic membrane (TM) when myringotomy was performed during acute otitis media. The early and long-lasting structural changes of the TM were studied in an animal model. Rats were inoculated with Streptococcus pneumoniae (PnC) type 3 in the bulla. When the infection was manifest, myringotomy was performed. On days 4 and 12, and 3 and 6 months after myringotomy, the TM status was checked by otomicroscopy and TMs were prepared for light and electron microscopy. Comparison was made with PnC-infected TMs that were not perforated, as well as myringotomized noninfected TMs. The infection resolved more slowly in myringotomized ears compared to PnC-infected ears that were left untouched. After 6 months, the pars tensa of the myringotomized infected ears was thickened and showed a disorganized collagen structure, compared with myringotomized noninfected ears, in which TMs were normalized. The PnC-infected TMs without myringotomy were completely normalized after 2 months. We conclude that a combination of bacterial infection and myringotomy causes long-lasting changes in TM structure. This impaired structure of the connective tissue could be of importance in chronic middle ear disease as a presumptive site for retraction and perforation of the TM.