Ethopharmacological analysis of behaviour of rats using variations of the elevated plus-maze
Ethopharmacological analysis of behaviour of rats using variations of the elevated plus-maze
复制标题
DOI：
10.1097/00008877-199711000-00011
复制
发表时间：
1997-11-01
影响因子：
1.6
通讯作者：
Brandao, ML
中科院分区：
文献类型：
--
作者：
Anseloni, VZ;Brandao, ML
除了允许更好地观察和记录啮齿动物的行为，我们已经表明，具有透明壁的高架十字迷宫与具有不透明壁的传统装置一样对抗焦虑药和致焦虑药的作用敏感。在这项研究中，我们扩展这些观察与动物行为药理学分析的大鼠在高架十字迷宫与透明的墙壁。因素分析的行为控制（盐水处理）大鼠的修改后的迷宫和一个标准的迷宫揭示了一个特征分布的行为类别中的六个因素。对于改良的迷宫，前三个因素，代表标准指数的焦虑，运动和风险评估，是类似的因素分布报告的这个和其他研究，使用标准的高架十字迷宫测试。不同的功能出现在因素4，与负载的rears在修改后的迷宫一起发生的百分比的中心时间和flat-back方法共同的两个迷宫。扫描和梳理，这是一般分组的因素5在标准迷宫，出现作为独立的行为元素加载的因素5和6，分别在修改后的迷宫。改良迷宫中行为的动物药理学分析表明，咪达唑仑（1和2 mg/kg）产生了抗焦虑选择性特征，而戊四唑（5和10 mg/kg）产生了抗焦虑样特征。这些结果表明，高架十字迷宫具有透明的墙壁，这可能是有益的不同方面的焦虑和抗焦虑药物的作用方式在大鼠的研究的独特功能。
Besides allowing better observation and recording of the behaviour of rodents, we have shown that the elevated plus-maze with transparent walls is as sensitive to anxiolytic and anxiogenic drug effects as a traditional apparatus with opaque walls. In this study we extend these observations with an ethopharmacological analysis of the behaviour of rats in the elevated plus-maze with transparent walls. A factor analysis of the behaviour of control (saline-treated) rats on the modified maze and on a standard maze revealed a characteristic distribution of the behavioural categories in six factors. For the modified maze, the first three factors, representing standard indices of anxiety, locomotion and risk assessment, were similar to the factor distribution reported by this and other studies using the standard elevated plus-maze test. Distinct features appeared on Factor 4, with the loading of rears in the modified maze together with the occurrence of the percentage of centre time and flat-back approach common to both mazes. Scanning and grooming, which are generally grouped on Factor 5 in the standard maze, appeared as isolated behavioural elements loading on Factors 5 and 6, respectively, in the modified maze. An ethopharmacological analysis of behaviour in the modified maze showed that midazolam (1 and 2 mg/kg) produced an anxioselective profile, whereas pentylenetetrazol (5 and 10 mg/kg) produced an anxiogenic-like profile. These results point to the distinct features of the elevated plus-maze with transparent walls, which may be beneficial in the study of the different facets of anxiety and the mode of action of anxiolytic drugs in rats.