Automated Quantitative Analysis of Airway Epithelial Cell Detachment Upon Fungal Challenge.
Automated Quantitative Analysis of Airway Epithelial Cell Detachment Upon Fungal Challenge.
复制标题
真菌攻击后气道上皮细胞脱离的自动定量分析。
DOI：
10.1007/978-1-0716-1182-1_16
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Rahman S
中科院分区：
文献类型：
--
作者：
Rahman S
宿主-病原体相互作用涉及宿主和病原体因子之间复杂的相互作用，导致宿主保护性免疫或疾病的建立。疾病进展的标志之一是宿主组织破坏。与机会性呼吸道真菌病原体烟曲霉相互作用的第一个宿主表面是气道上皮。揭示A.熏蒸对了解宿主中感染的建立和进展至关重要。虽然宿主细胞损伤可以在体外间接细胞裂解测定，在这里，我们描述了一个自动化的，简单的，低成本的测定直接可视化和量化上皮细胞系损伤后的挑战与A。烟熏。我们采用以前的特点组织noninvasiveA。fumigatus Δ pacC突变体，以证明相对于其亲本组织侵袭菌株的细胞损伤的定量差异。该检测方法易于扩大规模，用于多囊蚴突变体的高通量筛选，并可适应不同的宿主细胞系、不同的感染时间点、其他微生物的挑战以及药物或新型化合物。
Host–pathogen interactions involve a complex interplay between host and pathogen factors, resulting in either host protective immunity or establishment of disease. One of the hallmarks for disease progression is host tissue destruction. The first host surface to interact with the opportunistic respiratory fungal pathogen,Aspergillus fumigatus, is the airway epithelium. Unravelling the mechanisms involved in airway epithelial cell damage byA. fumigatusis essential to understanding the establishment and progression of infection in the host. Although host cell damage can be measured in vitro by indirect cell lysis assays, here, we describe an automated, simple, and low-cost assay to directly visualize and quantify epithelial cell line damage after challenge withA. fumigatus. We employ the previously characterized tissue noninvasiveA. fumigatus ΔpacCmutant to demonstrate the quantitative difference in cell damage relative to its parental tissue invasive strain. This assay is easily scaled up for high-throughput screening of multipleAspergillusmutants and can be adapted to suit diverse host cell lines, different time points of infection, challenge with other microbes, and drugs or novel compounds.
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert