Cutting edge:: Human B cell function is regulated by interaction with soluble CD14:: Opposite effects on IgG1 and IgE production
Cutting edge:: Human B cell function is regulated by interaction with soluble CD14:: Opposite effects on IgG1 and IgE production
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DOI：
10.4049/jimmunol.164.7.3480
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发表时间：
2000-04-01
影响因子：
4.4
通讯作者：
Labéta, MO
中科院分区：
文献类型：
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作者：
Arias, MA;Nores, JER;Labéta, MO
控制幼稚B细胞的活化、其增殖、Ag受体亲和力成熟、同种型转换及其作为记忆细胞或浆细胞的命运的机制尚未完全阐明。我们发现PBMC中24 - 60%的CD 19（+）细胞与可溶性CD 14（sCD 14）结合，扁桃体B细胞也与sCD 14结合，但优先与CD 38（-ve/low）细胞结合。sCD 14与B细胞的相互作用导致更高水平的IgG 1和显著抑制由活化的扁桃体B细胞和Ag刺激的PBMC产生的IgE。我们发现sCD 14干扰B细胞中的CD 40信号传导，抑制活化的B细胞产生IL-6，并增加T细胞上CD 40配体表达的动力学和幅度。连同先前报道的对T细胞的作用，这些发现将sCD 14定义为能够通过直接与T和B细胞相互作用来调节细胞和体液免疫应答的新型可溶性调节因子。
The mechanism(s) controlling activation of naive B cells, their proliferation, Ag receptor affinity maturation, isotype switching, and their fate as memory or plasma cells is not fully elucidated. Here we show that between 24 and 60% of CD19(+) cells in PBMC bind soluble CD14 (sCD14), Tonsillar B cells also bind sCD14, but preferentially the CD38(-ve/low) cells. Interaction of sCD14 with B cells resulted in higher levels of IgG1 and marked inhibition of IgE production by activated tonsillar B cells and Ag-stimulated PBMC. We found that sCD14 interfered with CD40 signaling in B cells, inhibited IL-6 production by activated B cells, and increased the kinetics and magnitude of CD40 ligand expression on T cells. Together with the previously reported effects on T cells, these findings define sCD14 as a novel soluble regulatory factor capable of modulating cellular and humoral immune responses by interacting directly with T and B cells.