Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells
Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells
复制标题
DOI：
10.1016/j.stem.2019.07.008
复制
发表时间：
2019-09-05
期刊：
影响因子：
23.9
通讯作者：
Koo, Bon-Kyoung
中科院分区：
文献类型：
--
作者：
Han, Seungmin;Fink, Juergen;Koo, Bon-Kyoung
胃本体上皮是胃肠道最厚的部分，并迅速翻转。在峡部和基底区已经提出了几个胃本体干细胞的标记物。然而，峡干细胞（IsthSCs）的身份和不同干细胞群体之间的相互作用仍然存在争议。在这里，基于无偏遗传标记和生物物理模型，我们发现体腺体被划分为两个独立的区域，缓慢循环的干细胞维持基部，积极循环的干细胞通过“间断”的中性漂移动力学过程维持坑-峡-颈区域。基于Stmn1和Ki67表达的独立谱系追踪证实，快速循环的IsthSCs维持了峡窝-峡颈区域。最后，使用单细胞RNA测序（RNA-seq）分析来确定单个循环IsthSC群体的分子身份和谱系关系。这些观察结果定义了IsthSCs的身份和功能行为。
The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relation-ship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.