Hypovirulence of the Phytopathogenic Fungus Botryosphaeria dothidea: Association with a Coinfecting Chrysovirus and a Partitivirus
Hypovirulence of the Phytopathogenic Fungus Botryosphaeria dothidea: Association with a Coinfecting Chrysovirus and a Partitivirus
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植物病原真菌 Botryosphaeria Dothidea 的低毒力：与共感染金病毒和部分病毒的关联
DOI：
10.1128/jvi.00538-14
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发表时间：
2014-07-01
影响因子：
5.4
通讯作者：
Wang, GuoPing
中科院分区：
文献类型：
--
作者：
Wang, LiPing;Jiang, JingJing;Wang, GuoPing
梨芽孢菌（Botryosphaeria dothidea）是引起梨树果实腐烂、叶茎年轮斑和枯死、茎溃疡病、茎死或粪亡和衰败的重要病原菌。7个双链rna （dsRNAs; dsRNAs 1 ~ 7，大小分别为3,654、2,773、2,597、2,574、1,823、1,623和511 bp）在一株dothidea分离物中被鉴定出，表现出生长减弱、毒力减弱和扇形表型。dsRNA的鉴定表明它们属于两种dsRNA分枝病毒。四个最大的dsRNAs （dsRNAs 1至4）是黄病毒科新成员（暂定名为Botryosphaeria dothidea chrysovirus 1 [BdCV1]）的基因组组成部分，这一观点得到了病毒粒子形态和推测的RNA依赖RNA聚合酶（RdRps）的系统发育分析的支持。另外两个dsRNAs （dsRNAs 5和dsRNAs 6）是Partitiviridae家族新成员（暂定名为Botryosphaeria dothidea partitivirus 1 [BdPV1]）的基因组成分，根据其RdRp的系统发育分析，将其置于与其他已建立的partitivirus属不同的分支中。最小的dsRNA dsRNA7似乎是BdPV1的非编码卫星RNA，基于其在BdPV1基因组片段中末端序列的保守性以及在水平传播后与BdPV1共分离。这是第一次报告了一种黄体病毒和一种局部病毒感染白僵菌，以及一种黄体病毒与一种植物致病真菌的低毒力有关。
Botryosphaeria dothidea is an important pathogenic fungus causing fruit rot, leaf and stem ring spots and dieback, stem canker, stem death or stool mortality, and decline of pear trees. Seven double-stranded RNAs (dsRNAs; dsRNAs 1 to 7 with sizes of 3,654, 2,773, 2,597, 2,574, 1,823, 1,623, and 511 bp, respectively) were identified in an isolate of B. dothidea exhibiting attenuated growth and virulence and a sectoring phenotype. Characterization of the dsRNAs revealed that they belong to two dsRNA mycoviruses. The four largest dsRNAs (dsRNAs 1 to 4) are the genomic components of a novel member of the family Chrysoviridae (tentatively designated Botryosphaeria dothidea chrysovirus 1 [BdCV1]), a view supported by the morphology of the virions and phylogenetic analysis of the putative RNA-dependent RNA polymerases (RdRps). Two other dsRNAs (dsRNAs 5 and 6) are the genomic components of a novel member of the family Partitiviridae (tentatively designated Botryosphaeria dothidea partitivirus 1 [BdPV1]), which is placed in a clade distinct from other established partitivirus genera on the basis of the phylogenetic analysis of its RdRp. The smallest dsRNA, dsRNA7, seems to be a noncoding satellite RNA of BdPV1 on the basis of the conservation of its terminal sequences in BdPV1 genomic segments and its cosegregation with BdPV1 after horizontal transmission. This is the first report of a chrysovirus and a partitivirus infecting B. dothidea and of a chrysovirus associated with the hypovirulence of a phytopathogenic fungus.