The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells.
The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells.
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DOI：
10.1016/j.bbrc.2013.02.123
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发表时间：
2013-05
影响因子：
3.1
通讯作者：
Yingyi Zhang;Yu Zhao;Leilei Li;Yu Shen;Xiao-li Cai;Xiao-dong Zhang;L. Ye
中科院分区：
文献类型：
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作者：
Yingyi Zhang;Yu Zhao;Leilei Li;Yu Shen;Xiao-li Cai;Xiao-dong Zhang;L. Ye
我们已经报道了癌蛋白B型肝炎病毒X相互作用蛋白（HBXIP）作为一种新的转录辅激活因子，促进乳腺癌细胞的增殖和迁移。以前，我们发现HBXIP能够激活乳腺癌细胞中的核因子-κB（NF-κB）。作为一种癌基因，血小板衍生生长因子β多肽（PDGFB）在肿瘤发生中起着至关重要的作用。在本研究中，我们发现HBXIP和PDGFB都在乳腺癌细胞系中高表达。有趣的是，HBXIP能够通过PDGFB增加NF-κB的转录活性，表明HBXIP与细胞中的PDGFB相关。此外，HBXIP能够在mRNA、蛋白和启动子水平上调PDGFB。然后，我们确定HBXIP通过激活转录因子Sp1刺激PDGFB的启动子。在功能上，HBXIP通过PDGFB在体外促进乳腺癌细胞的增殖。因此，我们得出结论，HBXIP上调PDGFB通过激活转录因子Sp1，以促进乳腺癌细胞的增殖。
We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-κB (NF-κB) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-κB through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.