The intracellular concentration of acetyl phosphate in Escherichia coli is sufficient for direct phosphorylation of two-component response regulators
The intracellular concentration of acetyl phosphate in Escherichia coli is sufficient for direct phosphorylation of two-component response regulators
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DOI：
10.1128/jb.00564-07
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发表时间：
2007-08-01
影响因子：
3.2
通讯作者：
Wolfe, Alan J.
中科院分区：
文献类型：
--
作者：
Klein, Adam H.;Shulla, Ana;Wolfe, Alan J.
乙酰磷酸是AckA-Pta途径的中间体，在大肠杆菌中作为全局信号。虽然乙酰磷酸通过双组分反应调节剂清楚地发出信号，但仍不清楚乙酰磷酸是否作为直接磷酸供体或通过间接机制发挥作用。我们使用二维薄层色谱法来测量整个生长曲线过程中乙酰磷酸、乙酰辅酶A、ATP和GTP的相对浓度。我们估计，在野生型细胞中乙酰磷酸的细胞内浓度达到至少3 mM，浓度足以激活双组分反应调节剂通过直接磷酰基转移。
Acetyl phosphate, the intermediate of the AckA-Pta pathway, acts as a global signal in Escherichia coli. Although acetyl phosphate clearly signals through two-component response regulators, it remains unclear whether acetyl phosphate acts as a direct phospho donor or functions through an indirect mechanism. We used two-dimensional thin-layer chromatography to measure the relative concentrations of acetyl phosphate, acetyl coenzyme A, ATP, and GTP over the course of the entire growth curve. We estimated that the intracellular concentration of acetyl phosphate in wild-type cells reaches at least 3 mM, a concentration sufficient to activate two-component response regulators via direct phosphoryl transfer.