Restricted diffusion and stability of carbachol-fluorescent nanospheres in-vivo.
Restricted diffusion and stability of carbachol-fluorescent nanospheres in-vivo.
复制标题
卡巴胆碱荧光纳米球体内的限制扩散和稳定性。
DOI：
10.1097/00001756-199105000-00008
复制
发表时间：
1991
期刊：
影响因子：
1.7
通讯作者：
Quattrochi,JJ
中科院分区：
文献类型：
--
作者：
Macklis,JD;Quattrochi,JJ
绘制药物激活后诱导行为状态变化的神经元群需要对药物注射部位进行离散定位，并且受到分子药物广泛扩散的限制。直径为50 ~ 100 nm的纳米球由于其相对较大的尺寸，可以显著降低扩散。用甲基14c对胆碱能激动剂碳醇进行放射性标记，并将其纳入乳胶纳米球递送系统（LNDS）中。我们定量比较了14c -碳二醇在这些纳米球内的扩散与游离的14c -碳二醇的扩散，结果表明，注射后10分钟至24小时，纳米球的径向扩散减少了大约10倍；大约90%的注入放射性分别被限制在大约100-150 μm和1400-1500 μm范围内。因此，在纳米球内掺入活性剂（如药物）大大提高了其体内递送的精度（这里约为体积的1000倍）。
MAPPING neuronal populations that induce behavioral state changes after pharmacological activation requires discrete localization of drug injection sites, and is limited by widespread diffusion of molecular drugs. Nanospheres with diameters of 50–100 nm can reduce diffusion significantly because of their relatively large sizes. The cholinergic agonist carbachol was radiolabeled with methyl 14 C and incorporated within a latex nanosphere delivery system (LNDS). We quantitatively compared diffusion of 14 C-carbachol within these nanospheres with that of free 14 C-carbachol, demonstrating approximately ten-fold reduced radial diffusion by nanospheres 10 min to 24 h post-injection; approximately 90% of injected radioactivity was restricted to regions within approximately 100–150 μm and 1400–1500 μm respectively. Thus, incorporation of active agents such as drugs within nanospheres dramatically increases the precision of their delivery in-vivo (here about 1000-fold by volume).