Phenolic Composition Analysis and Gene Expression in Developing Seeds of Yellow- and Black-seeded Brassica napus
Phenolic Composition Analysis and Gene Expression in Developing Seeds of Yellow- and Black-seeded Brassica napus
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黄籽和黑籽甘蓝型油菜种子发育过程中酚类成分分析及基因表达
DOI：
10.1111/jipb.12039
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发表时间：
2013-06-01
影响因子：
11.4
通讯作者：
Wang, Youping
中科院分区：
文献类型：
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作者：
Jiang, Jinjin;Shao, Yanlin;Wang, Youping
育种者重点关注黄籽甘蓝型油菜（油菜籽），因为与黑籽品种相比，其品质更好。此外，黄酮类化合物与这种油菜籽有关。在这项研究中，我们应用脂质色谱-电喷雾电离质谱（LC-ESI-MSn）来比较天然黑籽甘蓝型油菜和从白芥子-白芥子体杂交后代中选择的黄籽渗入系的发育种子中的黄酮类化合物。除了最丰富的酚类化合物（芥子碱和芥子酸）和 1, 2-二芥子酰葡萄糖外，还鉴定并定量了 16 种不同的黄酮类化合物，包括 (-)-表儿茶素、(-)-表儿茶素的五种单电荷低聚物（[DP 2]-、[DP 3]-、[DP 4]、[DP 2]-B2 和 [DP]） 2]-B5)、槲皮素、山奈酚、异鼠李素二己糖苷、山奈酚芥子酰三己糖苷、异鼠李素芥子酰三己糖苷、异鼠李素己糖苷硫酸盐和异鼠李素-3-O-葡萄糖苷。大多数黄酮类化合物随着种子发育而积累，而一些类黄酮在成熟过程中迅速减少。黄色种子材料中这些黄酮类化合物的含量低于黑色种子中的含量。此外，在两个油菜品种中观察到不溶性原花青素低聚物和可溶性酚酸的变化。通过定量逆转录聚合酶链反应分析发现了参与类黄酮途径的基因的转录组变化。与高效液相色谱分析确定的类黄酮变化一致，类黄酮生物合成途径中的大多数基因的表达也下调。
Breeders have focused on yellow-seeded Brassica napus (rapeseed) for its better quality compared with the black-seeded variety. Moreover, flavonoids have been associated with this kind of rapeseed. In this study, we applied lipid chromatography-electrospray ionization mass spectrometry (LC-ESI-MSn) to compare flavonoids in developing seeds of natural black-seeded B. napus and yellow-seeded introgression lines selected from progenies of B. napus-Sinapis alba somatic hybrids. Aside from the most abundant phenolic compounds (sinapine and sinapic acid) and 1, 2-disinapoylglucose, 16 different flavonoids were identified and quantified, including (-)-epicatechin, five monocharged oligomers of (-)-epicatechin ([DP 2]-, [DP 3]-, [DP 4] [DP 2]-B2 and [DP 2]-B5), quercetin, kaempferol, isorhamnetin-dihexoside, kaempferol-sinapoyl-trihexoside, isorhamnetin-sinapoyl-trihexoside, isorhamnetin-hexoside-sulfate, and isorhamnetin-3-O-glucoside. Most of the flavonoids accumulated with seed development, whereas some rapidly decreased during maturation. The content of these flavonoids were lower in the yellow-seeded materials than in the black seeds. In addition, variations of insoluble procyanidin oligomers and soluble phenolic acids were observed among both rapeseed varieties. Transcriptome changes of genes participating in the flavonoid pathway were discovered by quantitative reverse transcription polymerase chain reaction analysis. Consistent with flavonoid changes identified by high performance liquid chromatography analysis, the expression of most genes in the flavonoid biosynthetic pathway was also downregulated.