Efficient anther culture method of the japonica rice cultivar Koshihikari
Efficient anther culture method of the japonica rice cultivar Koshihikari
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DOI：
10.1270/jsbbs.50.197
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发表时间：
2000-09-01
期刊：
影响因子：
2.4
通讯作者：
Nagasawa, Y
中科院分区：
文献类型：
--
作者：
Daigen, M;Kawakami, O;Nagasawa, Y
以粳稻品种越光为杂交亲本选育高食味品种，利用花药培养方法在短时间内选育出了一个新品种，近年来已育成了几个品种。但越光花药培养效率较低。为了提高愈伤组织的培养效率，研制了适合越光愈伤组织培养的培养基。该培养基是R2培养基的改良，即，将R2培养基中的硝酸钾（KNO_3）和硫酸铵（（NH_4）（2）SO_4）稀释至1/5，加入5 mM天冬氨酸和5 mM谷氨酰胺，维生素用B_5培养基中的维生素代替，命名为DKN（稀释KNO_3）培养基。以DKN培养基为基本培养基，采用三步花药培养法对越光花药进行培养。在DKN培养基上，花药的绿色植株再生率比在1/2 R2培养基上高4.3倍（供试植株在绿色温室中培养）和21.4倍（供试植株在大田中培养）。说明三步花药培养法与DKN培养基相结合是越光花药培养的有效方法。
The japonica rice cultivar Koshihikari has been used as the crossing parent for breeding of high-eating quality cultivars, An anther culture method has been developed to breed a new rice cultivar with in a short period, and several cultivars have been released in recent years. However, the efficiency of anther culture of Koshihikari was low. We developed a culture medium suitable for Koshihikari callus culture to increase the anther culture efficiency. The medium was a modification of the R2 medium, i.e., the concentrations of potassium nitrate (KNO3) and ammonium sulfate ((NH4)(2)SO4) in the R2 medium were diluted to 1/5, 5 mM aspartic acid and 5 mM glutamine were added, and the vitamins were replaced by those of B5 medium, We designated the medium as DKN (dilute KNO3) medium. The anthers of Koshihikari were cultured by using the three-step anther culture method in which DKN medium was used as the basal medium. The green plant regeneration rate per anther on DKN medium was 4.3 times (donor plants cultivated in green house) and 21.4 times (donor plants grown in field) higher than those on 1/2R2 medium which had been used for rice breeding by anther culture. It is concluded that the combination of the three-step anther culture method and DKN medium is efficient for anther culture of Koshihikari.