Guanosine induces necrosis of cultured aortic endothelial cells.
Guanosine induces necrosis of cultured aortic endothelial cells.
复制标题
鸟苷诱导培养的主动脉内皮细胞坏死。
作者：
Z. Han;J. Wyche
我们观察到鸟苷处理培养的牛主动脉内皮（BAE）细胞可以抑制细胞的增殖和活力。向培养基中加入500 mumol/L鸟苷导致约90%的细胞增殖抑制。它还改变了BAE细胞的形态，从小鹅卵石样外观变成了巨大的煎饼样形态。在1 ~ 2.5mmol/L浓度范围内，鸟苷可抑制静止期BAE细胞的活力。用2 mmol/L鸟苷孵育细胞24小时，最大诱导细胞活力损失约80%。我们还比较了不同核苷对BAE细胞增殖和活力的影响，发现在适当的浓度范围内，只有鸟苷能够抑制细胞的增殖和活力。为了评估介导鸟苷细胞毒性的机制，我们分析了鸟苷处理的细胞中DNA的降解模式，发现细胞中发生随机DNA降解。因此，我们认为鸟苷处理BAE细胞可引起细胞坏死。
We have observed that treatment of cultured bovine aortic endothelial (BAE) cells with guanosine can inhibit the proliferation and viability of the cells. The addition of 500 mumol/L guanosine to the medium resulted in approximately 90% inhibition of cell proliferation. It also changed the morphology of BAE cells from having a small cobblestone-like appearance to a giant pancake-like morphology. At a concentration range of 1 to 2.5 mmol/L, guanosine inhibited the viability of quiescent BAE cells. Incubation of the cells with 2 mmol/L guanosine for 24 hours maximally induced the loss of cell viability by approximately 80%. We also compared the effects of different nucleosides on the proliferation and viability of BAE cells and found that at appropriate concentration ranges, only guanosine was able to inhibit the proliferation and viability of the cells. To assess the mechanism that mediates the cytotoxicity of guanosine, we analyzed the degradation pattern of DNA in guanosine-treated cells and found that random DNA degradation occurred in the cells. Thus, we suggest that treatment of BAE cells with guanosine induced cell necrosis.
影响因子：
5.8
作者：
BARRY, MA;BEHNKE, CA;EASTMAN, A
通讯作者：
EASTMAN, A