SPERMIDINE DEFICIENCY INCREASES +1 RIBOSOMAL FRAMESHIFTING EFFICIENCY AND INHIBITS TY1 RETROTRANSPOSITION IN SACCHAROMYCES-CEREVISIAE
SPERMIDINE DEFICIENCY INCREASES +1 RIBOSOMAL FRAMESHIFTING EFFICIENCY AND INHIBITS TY1 RETROTRANSPOSITION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.91.1.172
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发表时间：
1994-01-04
影响因子：
11.1
通讯作者：
TABOR, H
中科院分区：
文献类型：
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作者：
BALASUNDARAM, D;DINMAN, JD;TABOR, H
多胺与核酸相关的功能和蛋白质的生物合成有关。RNA序列可以特异性地引导核糖体在-1和+1方向上移动阅读框，可以用来探索控制翻译保真度的机制。我们通过体内实验研究了亚精胺对翻译保真度的影响。在体内实验中，β-半乳糖苷酶活性的变化依赖于酵母反转录病毒Ty+1和酵母双链RNA病毒L-A-1核糖体移码信号。在由于SPE2基因缺失而不能产生亚精胺的酿酒酵母sp2DELTA突变体中，核糖体+1的移码显著升高，而-1的移码没有改变。随着+1核糖体移码效率的提高，Ty1逆转录转座显著减少。
Polyamines have been implicated in nucleic acid-related functions and in protein biosynthesis. RNA sequences that specifically direct ribosomes to shift reading frame in the -1 and +1 directions may be used to probe the mechanisms controlling translational fidelity. We examined the effects of spermidine on translational fidelity by an in vivo assay in which changes in beta-galactosidase activity are dependent on yeast retrovirus Ty +1 and yeast double-stranded RNA virus L-A -1 ribosomal frameshifting signals. In spe2DELTA mutants of Saccharomyces cerevisiae, which cannot make spermidine as a result of a deletion in the SPE2 gene, there is a marked elevation in +1 but no change in -1 ribosomal frameshifting. The increase in +1 ribosomal frameshifting efficiency is accompanied by a striking decrease in Ty1 retrotransposition.