INCREASED EXPRESSION OF GENES FOR BASIC FIBROBLAST GROWTH-FACTOR AND TRANSFORMING GROWTH-FACTOR TYPE-BETA-2 IN HUMAN BENIGN PROSTATIC HYPERPLASIA
INCREASED EXPRESSION OF GENES FOR BASIC FIBROBLAST GROWTH-FACTOR AND TRANSFORMING GROWTH-FACTOR TYPE-BETA-2 IN HUMAN BENIGN PROSTATIC HYPERPLASIA
复制标题
DOI：
10.1002/pros.2990160108
复制
发表时间：
1990-01-01
期刊：
影响因子：
2.8
通讯作者：
HATANAKA, M
中科院分区：
文献类型：
--
作者：
MORI, H;MAKI, M;HATANAKA, M
现在人们普遍认为，雄激素以外的因素在前列腺的正常和异常生长中起着关键作用，包括人类良性前列腺增生症(BPH)。通过Northern印迹分析，我们检测了人正常和良性增生前列腺组织中碱性成纤维细胞生长因子、酸性成纤维细胞生长因子、转化生长因子β2(转化生长因子β2)、转化生长因子β1和表皮生长因子的表达。在所有被检测的前列腺中均可检测到碱性成纤维细胞生长因子mRNAs。此外，BPH组织中碱性成纤维细胞生长因子的表达水平显著高于正常前列腺组织。酸性成纤维细胞生长因子转录本除1例良性前列腺增生症外，均未被检测到。尽管这两种转化生长因子在所有样本中都有表达，但与正常前列腺组织相比，转化生长因子-β2在良性前列腺组织中的表达水平显著增加，而转化生长因子-β1则不表达。所有受检的前列腺均未见EGF的表达。这些发现表明，前列腺局部产生的特定生长因子(碱性成纤维细胞生长因子和转化生长因子α2)可能与BPH的发生有关。
It is now widely accepted that factors other than androgen are crucial in the normal and abnormal growth of the prostate, including human benign prostatic hyperplasia (BPH). Using a Northern blot analysis, we examined human normal and benign hyperplastic prostates for expressions of basic fibroblast growth factor (FGF), acidic FGF, transforming growth factor type .beta.2 (TGF-.beta.2), TGF-.beta.1, and epidermal growth factor (EGF). Basic FGF mRNAs were detectable in all the prostates examined. In addition, levels of basic FGF expression were significantly higher in BPH than in normal prostate. Acidic FGF transcripts were undetectable except in one case of BPH. Although both TGFs were expressed in all the samples, TGF-.beta.2 showed significantly increased levels of expression in BPH as compared to those in normal prostate, while TGF-.beta.1 did not. No EGF was expressed in any of the prostates examined. These findings suggest that specific growth factors (basic FGF and TGF-.alpha.2) produced locally in the prostate may be involved in BPH development.