In Vitro Behavior of Human Adipose Tissue-Derived Stem Cells on Poly(ε-caprolactone) Film for Bone Tissue Engineering Applications.
In Vitro Behavior of Human Adipose Tissue-Derived Stem Cells on Poly(ε-caprolactone) Film for Bone Tissue Engineering Applications.
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DOI：
10.1155/2015/323571
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发表时间：
2015
影响因子：
--
通讯作者：
Brandi ML
中科院分区：
文献类型：
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作者：
Romagnoli C;Zonefrati R;Galli G;Puppi D;Pirosa A;Chiellini F;Martelli FS;Tanini A;Brandi ML
骨组织工程是一个新兴的领域，代表了科学家和临床医生最令人兴奋的挑战之一。将间充质干细胞和支架组合以产生工程化组织的可能性引起了人们对与能够促进和再生骨组织的骨祖细胞组合的大量生物材料的关注。人类脂肪组织被官方认为是一种容易获得的间充质干细胞（AMSC）来源，这是用于组织再生医学的重要因素。在这项研究中，我们分析了来自人脂肪组织的克隆有限细胞系接种在聚（ε-己内酯）（PCL）膜，通过溶剂浇铸制备的行为。PCL聚合物因其良好的生物相容性、生物降解性和机械性能而被选择。我们观察到AMSC能够粘附在生物材料上，并在整个实验期间保持活力。此外，我们表明，AMSC的增殖过程和成骨活性保持在生物膜上，表明所选的生物材料确保细胞定植和细胞外矿化基质的发展。本研究的结果表明，AMSCs和PCL膜可以作为一个合适的模型，以支持新骨的再生，为未来的组织工程策略。
Bone tissue engineering is an emerging field, representing one of the most exciting challenges for scientists and clinicians. The possibility of combining mesenchymal stem cells and scaffolds to create engineered tissues has brought attention to a large variety of biomaterials in combination with osteoprogenitor cells able to promote and regenerate bone tissue. Human adipose tissue is officially recognized as an easily accessible source of mesenchymal stem cells (AMSCs), a significant factor for use in tissue regenerative medicine. In this study, we analyze the behavior of a clonal finite cell line derived from human adipose tissue seeded on poly(ε-caprolactone) (PCL) film, prepared by solvent casting. PCL polymer is chosen for its good biocompatibility, biodegradability, and mechanical properties. We observe that AMSCs are able to adhere to the biomaterial and remain viable for the entire experimental period. Moreover, we show that the proliferation process and osteogenic activity of AMSCs are maintained on the biofilm, demonstrating that the selected biomaterial ensures cell colonization and the development of an extracellular mineralized matrix. The results of this study highlight that AMSCs and PCL film can be used as a suitable model to support regeneration of new bone for future tissue engineering strategies.