Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes.
Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes.
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DOI：
10.1186/s12864-022-08516-6
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发表时间：
2022-04-18
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
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作者：
华山新竹因其具有抗多种病害（条锈病、叶锈病、全食病、白粉病）的基因以及良好的农艺性状，长期以来被用作小麦品种改良的遗传资源。然而，华山松的高分辨率荧光原位杂交（FISH）核型仍然无法获得。为了开发华山松的染色体特异性 FISH 标记，使用重复序列（包括 pSc119.2、pTa535、pTa713、pAs1、(AAC)5、(CTT)12、pSc200、pTa71A-2 和 Oligo-44）进行 FISH 分析。结果表明，pSc200、pTa71A-2和Oligo-44探针组合可以清晰地识别两种华山松种质中的所有Ns基因组染色体。通过FISH染色阐明了华山黑麦个体染色体与普通小麦染色体的同源关系。标记验证分析表明，pSc200、pTa71A-2 和 Oligo-44 组合进行 FISH 分析可以区分华山松 Ns 基因组染色体与小麦染色体，以及所有染色体（除 4N 外）与携带 St、E、V、I、P 和 R 基因组的二倍体小麦近缘种的染色体。此外，该探针还可用于区分华山松所有同源群中的Ns基因组染色体与芥花和大多数含有Ns基因组的羊草属物种中的相应染色体。此外，六种小麦-P。使用新开发的 FISH 标记对华山虫染色体附加系（即 2Ns、3Ns、4Ns、7Ns 染色体和染色体片段）进行了表征。因此，这些探针可以快速、准确地检测小麦背景中的华山松外来染色体。本研究建立的FISH核型为小麦遗传改良中华山黑麦染色体的高效鉴定奠定了坚实的基础。
Psathyrostachys huashanica Keng has long been used as a genetic resource for improving wheat cultivar because of its genes mediating the resistance to various diseases (stripe rust, leaf rust, take-all, and powdery mildew) as well as its desirable agronomic traits. However, a high-resolution fluorescence in situ hybridization (FISH) karyotype of P. huashanica remains unavailable. To develop chromosome-specific FISH markers for P. huashanica, repetitive sequences, including pSc119.2, pTa535, pTa713, pAs1, (AAC)5, (CTT)12, pSc200, pTa71A-2, and Oligo-44 were used for a FISH analysis. The results indicated that the combination of pSc200, pTa71A-2 and Oligo-44 probes can clearly identify all Ns genomic chromosomes in the two P. huashanica germplasms. The homoeologous relationships between individual P. huashanica chromosomes and common wheat chromosomes were clarified by FISH painting. Marker validation analyses revealed that the combination of pSc200, pTa71A-2, and Oligo-44 for a FISH analysis can distinguish the P. huashanica Ns-genome chromosomes from wheat chromosomes, as well as all chromosomes (except 4Ns) from the chromosomes of diploid wheat relatives carrying St, E, V, I, P and R genomes. Additionally, the probes were applicable for discriminating between the P. huashanica Ns-genome chromosomes in all homologous groups and the corresponding chromosomes in Psathyrostachys juncea and most Leymus species containing the Ns genome. Furthermore, six wheat–P. huashanica chromosome addition lines (i.e., 2Ns, 3Ns, 4Ns, 7Ns chromosomes and chromosomal segments) were characterized using the newly developed FISH markers. Thus, these probes can rapidly and precisely detect P. huashanica alien chromosomes in the wheat background. The FISH karyotype established in this study lays a solid foundation for the efficient identification of P. huashanica chromosomes in wheat genetic improvement programs.
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影响因子：
0.9
作者：
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通讯作者：
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作者：
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