Using Color Infrared Imagery to Detect Sooty Mold and Fungal Pathogens of Glasshouse-propagated Plants
Using Color Infrared Imagery to Detect Sooty Mold and Fungal Pathogens of Glasshouse-propagated Plants
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使用彩色红外图像检测温室繁殖植物的煤烟霉菌和真菌病原体
DOI：
10.21273/hortsci.43.5.1485
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发表时间：
2008
期刊：
影响因子：
1.9
通讯作者：
C. Little
中科院分区：
文献类型：
--
作者：
K. Summy;C. Little
真菌是制约温室植物最佳产量和质量的主要生物因素。当植物被烟霉（Capnodiumspp.）侵染或被病原体感染时，电磁波谱的反射波长就会发生改变。对三叶橙（Poncirus trifoliata）、酸橙（Citrus aurantium）、‘Valencia’橙（C. sinensis）和‘Bo’树（Ficus religiosa）的对照、蜜露包覆和黑霉侵染的树苗和单叶进行了光谱辐射测量和彩色红外（CIR）成像。在温室条件下对感染柑桔分枝杆菌（油斑）的葡萄柚幼树和单叶进行了成像。同样，分析了甜瓜叶片白粉病（白粉病）严重程度的高低。当检测单个叶片时，所有真菌生物胁迫通常会产生可变的光谱反射率数据，特别是在蓝色（450 nm）和绿色（550 nm）波长；然而，随着应力的增加，红光（650 nm）的值有增加的趋势，近红外（850 nm）的值有下降的趋势。与健康对照相比，胁迫下全叶和单叶近红外/红像比值显著降低（P < 0.05）。虫蜜露的积累（发生在黑霉侵染前）显著提高了“瓦伦西亚”橙的近红外反射率值和近红外/红比值（P < 0.05），以及“博”树叶片和单叶的近红外/红比值（P < 0.05）。图像采集和增强技术可能在现有基础设施和高植物种群需要高通量数据分析和识别生物应激源的大规模生产温室中有用。
Fungi are major biotic constraints for optimum production and quality of glasshouse plants.Whenplants areinfested withsootymold (Capnodiumspp.) or infected with pathogens, the reflected wavelengths of the electromagnetic spectrum are altered. Spectroradiometric measurements and color infrared (CIR) images of control, honey- dew-coated,andsooty mold-infestedsaplingsandindividualleaves fromtrifoliateorange (Poncirus trifoliata), sour orange (Citrus aurantium), 'Valencia' orange (C. sinensis), and 'Bo' tree (Ficus religiosa) were obtained. Grapefruit saplings and individual leaves infected with Mycosphaerella citri (greasy spot) were imaged under glasshouse con- ditions. Similarly, muskmelon foliage showing low and high levels of powdery mildew (Sphaerotheca fuliginea) disease severity were analyzed. When examining individual leaves, all fungal biotic stressors generally resulted in variable spectral reflectance data, especially in the blue (450 nm) and green (550 nm) wavelengths; however, values in the red (650 nm) tended to increase and values in the near-IR (850 nm) tended to decrease with stress. Near-IR/red image ratios were significantly reduced (P < 0.05) in stressed whole plant foliage and individual leaves relative to healthy controls. The accumulation of insect honeydew (which occurs before sooty mold infestation) significantly increased (P < 0.05) near-IR reflectance values and near-IR/red ratios in 'Valencia' orange and near-IR/ratios in 'Bo' tree foliage and individual leaves. Image acquisition and enhancement techniques may prove useful in large-scale production greenhouses where existing infrastructure and high plant populations require high throughput data analysis and identification of biotic stressors.