EXPONENTIAL INCREASES OF RNA VIRUS FITNESS DURING LARGE POPULATION TRANSMISSIONS
EXPONENTIAL INCREASES OF RNA VIRUS FITNESS DURING LARGE POPULATION TRANSMISSIONS
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DOI：
10.1073/pnas.92.13.5841
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发表时间：
1995-06-20
影响因子：
11.1
通讯作者：
HOLLAND, JJ
中科院分区：
文献类型：
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作者：
NOVELLA, IS;DUARTE, EA;HOLLAND, JJ
RNA病毒表现出的巨大适应性是其高突变率的结果。在这里，我们研究了在细胞培养中大量病毒群体重复转移过程中病毒适应度获得的动力学。结果总是显示适合度呈指数增长。低适应度克隆表现出有规律的增长，观察到指数进化改善的双相时期，而中性克隆表现出单相动力学。这些结果对RNA病毒流行病学、减毒活疫苗的最佳处理和常规实验室程序具有重要意义。
The great adaptability shown by RNA viruses is a consequence of their high mutation rates, Here we investigate the kinetics of virus fitness gains during repeated transfers of large virus populations in cell culture. Results always show that fitness increases exponentially. Low fitness clones exhibit regular increases observed as biphasic periods of exponential evolutionary improvement, while neutral clones show monophasic kinetics. These results are significant for RNA virus epidemiology, optimal handling of attenuated live virus vaccines, and routine laboratory procedures.