FKF1F-BOX protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis
FKF1F-BOX protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis
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DOI：
10.1126/science.1110586
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发表时间：
2005-07-08
期刊：
影响因子：
56.9
通讯作者：
Kay, SA
中科院分区：
文献类型：
--
作者：
Imaizumi, T;Schultz, TF;Kay, SA
CONSTANS（CO）表达和活性的时间控制是拟南芥光周期开花的关键机制。黄素结合，KELCH重复，F-BOX 1（FKF 1）蛋白调节CO转录，尽管分子机制尚不清楚。我们在这里证明，FKF 1控制的Dof转录因子，细胞周期自由度因子1（CDF 1）的稳定性。FKF 1与CDF 1发生物理相互作用，并且CDF 1蛋白在fkf 1突变体中更稳定。具有升高水平的CDF 1的植物开花较晚并且具有降低的CO表达。CDF 1和CO在相同的组织中表达，并且CDF 1与CO启动子结合。因此，FKF 1通过降解一氧化碳转录抑制因子CDF 1来部分控制一氧化碳的日常表达。
The temporal control of CONSTANS (CO) expression and activity is a key mechanism in photoperiodic flowering in Arabidopsis. FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1) protein regulates CO transcription, although the molecular mechanism is unknown. We demonstrate here that FKF1 controls the stability of a Dof transcription factor, CYCLING DOF FACTOR 1 (CDF1). FKF1 physically interacts with CDF1, and CDF1 protein is more stable in fkf1 mutants. Plants with elevated levels of CDF1 flower late and have reduced expression of CO. CDF1 and CO are expressed in the same tissues, and CDF1 binds to the CO promoter. Thus, FKF1 controls daily CO expression in part by degrading CDF1, a repressor of CO transcription.