Expansion, Reexpansion, and Recall-Like Expansion of Vγ2Vδ2 T Cells in Smallpox Vaccination and Monkeypox Virus Infection
Expansion, Reexpansion, and Recall-Like Expansion of Vγ2Vδ2 T Cells in Smallpox Vaccination and Monkeypox Virus Infection
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DOI：
10.1128/jvi.00689-09
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发表时间：
2009-11-15
影响因子：
5.4
通讯作者：
Chen, Zheng W.
中科院分区：
文献类型：
--
作者：
Shao, Lingyun;Huang, Dan;Chen, Zheng W.
关于天花/猴痘中T细胞反应的体内动力学知之甚少。我们发现，猕猴V γ 2 V δ 2 T细胞在天花疫苗免疫后经历了3周的扩增，并在MPV攻击后显示与无菌抗猴痘病毒（抗MPV）免疫相关的简单再扩增。病毒活化的V γ 2 V δ 2 T细胞在磷酸化抗原刺激后表现出γ干扰素产生效应子功能。令人惊讶的是，像α β T细胞一样，在MPV攻击后，牛痘病毒/西多福韦共接种的猕猴中的次最佳引发的V γ 2 V δ 2 T细胞出现了主要的召回样扩增。最后，V γ 2 V δ 2 T细胞定位于发炎的肺组织中用于潜在调节。我们的研究提供了第一个体内证据，即病毒，尽管它们不能产生外源性磷酸化抗原，但可以诱导V γ 2 V δ 2 T细胞的扩增、再扩增和回忆样扩增，并刺激它们的抗微生物细胞因子应答。
Little is known about the in vivo kinetics of T-cell responses in smallpox/monkeypox. We showed that macaque V gamma 2V delta 2 T cells underwent 3-week-long expansion after smallpox vaccine immunization and displayed simple reexpansion in association with sterile anti-monkeypox virus (anti-MPV) immunity after MPV challenge. Virus-activated V gamma 2V delta 2 T cells exhibited gamma interferon-producing effector function after phosphoantigen stimulation. Surprisingly, like alpha beta T cells, suboptimally primed V gamma 2V delta 2 T cells in vaccinia virus/cidofovir-covaccinated macaques mounted major recall-like expansion after MPV challenge. Finally, V gamma 2V delta 2 T cells localized in inflamed lung tissues for potential regulation. Our studies provide the first in vivo evidence that viruses, despite their inability to produce exogenous phosphoantigen, can induce expansion, reexpansion, and recall-like expansion of V gamma 2V delta 2 T cells and stimulate their antimicrobial cytokine response.