Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex
Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex
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DOI：
10.1126/science.1153445
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发表时间：
2008-02-29
期刊：
影响因子：
56.9
通讯作者：
Lerner, Richard A.
中科院分区：
文献类型：
--
作者：
Debler, Erik W.;Kaufmann, Gunnar F.;Lerner, Richard A.
针对反式二苯乙烯的蓝色发光抗体EP 2 - 19 G2具有前所未有的产生明亮发光的能力，并已在各种应用中用作生物传感器。我们表明，延长发光不是二苯乙烯荧光。相反，发射物质是阴离子芪和阳离子平行π堆叠色氨酸的电荷转移激发复合物。在电荷重组时，这种复合物产生异常明亮的蓝光。复合物的形成是通过深入穿透的配体结合口袋实现的，而这又是由抗体的两个可变结构域之间的非经典界面引起的。
The blue- emissive antibody EP2-19G2 that has been elicited against trans- stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge- transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.