THE E-TEST AND CAMPYLOBACTER-JEJUNI
THE E-TEST AND CAMPYLOBACTER-JEJUNI
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DOI：
10.1016/0732-8893(92)90092-8
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发表时间：
1992-07-01
影响因子：
2.9
通讯作者：
BAKER, CN
中科院分区：
文献类型：
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作者：
BAKER, CN
E-Test是最近引入的用于进行抗菌药物敏感性试验的方法。我们比较了E-测试肉汤微量稀释试验和标准琼脂稀释试验，使用5种抗菌剂对55个临床分离的空肠弯曲菌在美国的11个地点（组1）。后来，我们选择了30个菌株（组2），这是比原来的调查菌株更耐药。对两组微生物进行了红霉素、四环素和环丙沙星检测。当在+/- 1 log 2稀释度下相互比较三种检测方法时，E-检验给出了最佳的总体一致性，所有菌株的85%在可接受限度内。使用当前NCCLS指南折点，红霉素（首选药物）结果的类别解释是一个问题。对于E-Test和琼脂稀释法，82%的菌株属于中间类别;但使用肉汤微量稀释法，仅16.4%的分离株被解释为中间。如果敏感类别断点提高到小于或等于2 μ g/ml，则只有3%的C.空肠分离株可通过三种方法中的任一种解释为中间体。我们倾向于对C.空肠是E-Test或琼脂稀释在42 ℃下16小时。
The E-Test is a recently introduced method for performing antimicrobial susceptibility tests. We compared the E-Test to the broth microdilution test and to the standard agar dilution test by using five antimicrobial agents tested against 55 clinical isolates of Campylobacter jejuni from 11 locations in USA (group 1). Later, we selected 30 strains (group 2), which were more resistant than the original survey isolates. Erythromycin, tetracycline, and ciprofloxacin were tested on both groups of organisms. When the three test methods were compared with each other at +/- 1 log2 dilution, the E-test gave the best overall agreement, with 85% of all strains being within acceptable limits. Category interpretation of erythromycin (drug of choice) results was a problem using current NCCLS guideline breakpoints. For the E-Test and the agar dilution method, 82% of the strains were in the intermediate category; but with the broth microdilution method, only 16.4% of the isolates were interpreted as intermediate. If the susceptible category breakpoint was raised to less-than-or-equal-to 2-mu-g/ml, then only 3% of the C. jejuni isolates would be interpreted as intermediate by any of the three methods. Our preference for antimicrobial susceptibility testing of C. jejuni is either E-Test or agar dilution at 42-degrees-C for 16 hr.