Evaluation of the association of nine Helicobacter pylori virulence factors with strains involved in low-grade gastric mucosa-associated lymphoid tissue lymphoma
Evaluation of the association of nine Helicobacter pylori virulence factors with strains involved in low-grade gastric mucosa-associated lymphoid tissue lymphoma
复制标题
DOI：
10.1128/iai.72.2.880-888.2004
复制
发表时间：
2004-02-01
影响因子：
3.1
通讯作者：
Mégraud, F
中科院分区：
文献类型：
--
作者：
Lehours, P;Ménard, A;Mégraud, F
幽门螺杆菌与两种恶性疾病的发生有关：胃腺癌和胃粘膜相关淋巴组织（MALT）淋巴瘤。虽然cag毒力岛，特别是cagA基因与腺癌有关，但有关H.参与低度胃MALT淋巴瘤的幽门致病因素是可用的。本研究的目的是分析7种H. pylori毒力因子（cagA、cagE、vacA、iceA、babA、hopQ和oipA）和两种新的粘附素。(sabA和hopZ）的43 H. pylori菌株分离自低度胃MALT淋巴瘤患者，39株分离自年龄匹配的胃炎患者。我们的研究结果表明，单独考虑，测试的9个基因中没有一个可以被认为与MALT菌株相关，并允许我们得出结论，MALT发病机制与更多的促炎性H。pylori菌株。我们证明，在感染携带iceA1等位基因、sabA功能状态和hopZ“关闭”状态的菌株的患者中，发生MALT淋巴瘤的几率高10倍。然而，这种菌株的低流行率（43种MALT菌株中的10种）使得这种三重关联成为MALT菌株的低敏感性标记。我们的数据证实了H. pylori毒力因子之间相互关联。如果H.幽门螺杆菌在MALT淋巴瘤中的作用已被确立，胃淋巴瘤发生的病理机制仍有待确定。
Helicobacter pylori has been associated with the development of two malignant diseases: gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Although the cag pathogenicity island, especially the cagA gene, has been linked with adenocarcinoma, few data concerning H. pylori pathogenic factors involved in low-grade gastric MALT lymphoma are available. The goal of this study was to analyze the prevalence of and correlation between genes coding for seven H. pylori virulence factors (cagA, cagE, vacA, iceA, babA, hopQ, and oipA) and two novel adhesins. (sabA and hopZ) by comparing a collection of 43 H. pylori strains isolated from patients with low-grade gastric MALT lymphoma to 39 strains isolated from age-matched patients with gastritis only. Our results show that taken individually, none of the nine genes tested can be considered associated with MALT strains and allow us to conclude that MALT pathogenesis is not linked with more proinflammatory H. pylori strains. We demonstrated that in patients infected with strains harboring the iceA1 allele, sabA functional status, and hopZ "off" status, the odds of developing a MALT lymphoma were 10 times higher. However, the low prevalence of such strains (10 of 43 MALT strains) renders this triple association a low-sensitivity marker for MALT strains. Our data confirmed that H. pylori virulence factors are correlated with one another. If the involvement of H. pylori in MALT lymphoma is well established, the pathomechanism by which gastric lymphoma occurs remains to be identified.