Is Mycobacterium tuberculosis a closer relative to Gram-positive or Gram-negative bacterial pathogens?
Is Mycobacterium tuberculosis a closer relative to Gram-positive or Gram-negative bacterial pathogens?
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DOI：
10.1054/tube.2002.0328
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发表时间：
2002-01-01
期刊：
影响因子：
3.2
通讯作者：
Fu-Liu, CS
中科院分区：
文献类型：
--
作者：
Fu, LM;Fu-Liu, CS
结核分枝杆菌相对于其他细菌的系统发育地位存在争议。其细胞壁同时具有革兰氏阳性菌和革兰氏阴性菌的特征。在16S核糖体RNA序列比对的细菌系统发育标准参考中，结核分枝杆菌属于高G+C革兰氏阳性菌，与枯草芽孢杆菌等低G+C革兰氏阳性菌组成一个单系群。一些分析表明，这两个群体之间没有特殊的关系。结核分枝杆菌全基因组序列的获得使我们能够从基因组角度重新审视这一问题，因为基于基因组的系统发育可能比分子树更能代表整个生物体的进化史。在基于保守基因内容构建的基因组树中，结核分枝杆菌与革兰氏阴性菌的亲缘关系比与革兰氏阳性菌的亲缘关系更大，这从最近的祖先单位之间的进化距离反映出来。这一结论可能得到另一项分析的支持，该分析表明，与革兰氏阳性菌相比，结核分枝杆菌与革兰氏阴性菌，特别是大肠埃希菌和铜绿假单胞菌具有相对更多的能量产生和转换的同源基因。(C)2002爱思唯尔科学有限公司。保留所有权利。
dThe phylogenetic position of Mycobacterium tuberculosis relative to other bacteria is controversial. Its cell wall has characteristics of both Gram-positive and Gram-negative bacteria. In the standard reference of bacterial phylogeny based on 16S ribosomal RNA sequence comparison, M. tuberculosis belongs to the high G+C Gram-positive bacteria that form a monophyletic group with the low G+C Gram-positive bacteria such as Bacillus subtilis. Some analyses indicate no particular relationship between these two groups. The availability of the complete genome sequence of M. tuberculosis allows us to reexamine this issue from genomic perspectives, as genome-based phylogenies may be more representative of the evolutionary history of whole organisms than molecular trees. In the genome tree constructed based on conserved gene content, M. tuberculosis is more related to Gram-negative than to Gram-positive bacteria as reflected by the evolutionary distance between nearest ancestral units. This conclusion may be supported by another analysis showing that M. tuberculosis shares relatively more orthologous genes for energy production and conversion with Gram-negative bacteria, in particular, Escherichia coli and Pseudomonas aeruginosa, than with Gram-positive bacteria. (C) 2002 Elsevier Science Ltd. All rights reserved.