THE EXPRESSION OF V-PRE-B/LAMBDA-5 SURROGATE LIGHT-CHAIN IN EARLY BONE-MARROW PRECURSOR B-CELLS OF NORMAL AND B-CELL-DEFICIENT MUTANT MICE
THE EXPRESSION OF V-PRE-B/LAMBDA-5 SURROGATE LIGHT-CHAIN IN EARLY BONE-MARROW PRECURSOR B-CELLS OF NORMAL AND B-CELL-DEFICIENT MUTANT MICE
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DOI：
10.1016/0092-8674(94)90241-0
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发表时间：
1994-04-08
期刊：
影响因子：
64.5
通讯作者：
MELCHERS, F
中科院分区：
文献类型：
--
作者：
KARASUYAMA, H;ROLINK, A;MELCHERS, F
分析正常和B细胞缺陷突变小鼠骨髓中的前体B（前B）细胞的V-前B/λ 5替代轻链（SL）表达。SL的表面表达局限于前B细胞发育的早期阶段（pro-B和pre-B-I），并且一旦产生C重链（pH）就变得不可检测。细胞周期分析显示，细胞质mu H+大细胞（大pre-B-II），类似于30%的细胞质中共表达SL，是最活跃的周期，而细胞质mu H+小细胞（小pre-b-II）是SL（-），而不是在周期中。对B细胞缺陷小鼠中的前B细胞的分析表明，大的前B-II期是选择和扩增携带功能重排的μ H基因的细胞的关键步骤。
Precursor B (pre-B) cells in bone marrow of normal and B cell-deficient mutant mice were analyzed for the expression of V-pre-B/lambda 5 surrogate light chain (SL). The surface expression of SL is confined to the early stages (pro-B and pre-B-I) of pre-B cell development and becomes undetectable once C heavy chain (pH) is produced. The cell-cycle analysis revealed that cytoplasmic mu H+ large cells (large pre-B-II), similar to 30% of which coexpressed SL in the cytoplasm, were most actively cycling, whereas cytoplasmic mu H+ small cells (small pre-b-II) were SL(-) and not in cycle. The analysis of pre-B cells in B cell-deficient mice suggests that the large pre-B-II stage is a critical step for the selection and amplification of cells carrying functionally rearranged mu H genes.