Single-cell genomics revolutionizes plant development studies across scales.
Single-cell genomics revolutionizes plant development studies across scales.
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DOI：
10.1242/dev.200179
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发表时间：
2022-03
期刊：
影响因子：
4.6
通讯作者：
Mingyuan Zhu;Isaiah W. Taylor;P. Benfey
中科院分区：
文献类型：
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作者：
Mingyuan Zhu;Isaiah W. Taylor;P. Benfey
通过单细胞基因组学的透镜了解组织、器官和整个生物体的发育，彻底改变了发育生物学。虽然单细胞转录组学已经在动物系统中开创，但从实验的角度来看，植物发育具有一些明显的优势：细胞不会相互迁移，新器官的形成（叶，根，花等）。胚胎期后从称为分生组织的持久性干细胞群继续。有一段时间，植物研究落后于基于动物或细胞培养的单细胞方法，这主要是由于难以将植物细胞从其坚硬的细胞壁中分离出来。近年来，植物单细胞和单核分离技术的迅速发展为植物细胞分离提供了一个新的实验途径。这产生了跨组织类型和物种的信息的迅速扩大的多样性，伴随着方法的创造性发展。在这个简短的聚焦中，我们强调了一些技术发展以及它们如何导致在各种植物器官中分析单细胞基因组学。我们还强调了单细胞基因组学在揭示植物器官内不同细胞类型之间的发育轨迹方面的贡献。此外，我们还致力于在单细胞水平上对组织和器官进行比较分析。单细胞基因组学开始产生有关植物器官如何从干细胞群体中出现的全面信息。
Understanding the development of tissues, organs and entire organisms through the lens of single-cell genomics has revolutionized developmental biology. Although single-cell transcriptomics has been pioneered in animal systems, from an experimental perspective, plant development holds some distinct advantages: cells do not migrate in relation to one another, and new organ formation (of leaves, roots, flowers, etc.) continues post-embryonically from persistent stem cell populations known as meristems. For a time, plant studies lagged behind animal or cell culture-based, single-cell approaches, largely owing to the difficulty in dissociating plant cells from their rigid cell walls. Recent intensive development of single-cell and single-nucleus isolation techniques across plant species has opened up a wide range of experimental approaches. This has produced a rapidly expanding diversity of information across tissue types and species, concomitant with the creative development of methods. In this brief Spotlight, we highlight some of the technical developments and how they have led to profiling single-cell genomics in various plant organs. We also emphasize the contribution of single-cell genomics in revealing developmental trajectories among different cell types within plant organs. Furthermore, we present efforts toward comparative analysis of tissues and organs at a single-cell level. Single-cell genomics is beginning to generate comprehensive information relating to how plant organs emerge from stem cell populations.