Characterizing Soluble Protein Aggregates Using Native Mass Spectrometry Coupled with Temperature-Controlled Electrospray Ionization and Size-Exclusion Chromatography
Characterizing Soluble Protein Aggregates Using Native Mass Spectrometry Coupled with Temperature-Controlled Electrospray Ionization and Size-Exclusion Chromatography
复制标题
使用天然质谱法结合温控电喷雾电离和尺寸排阻色谱法表征可溶性蛋白质聚集体
DOI：
10.1007/978-1-0716-1859-2_27
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发表时间：
2022
影响因子：
--
通讯作者：
Wang, G.
中科院分区：
文献类型：
--
作者：
Muneeruddin, K.;Kaltashov, I.A.;Wang, G.
可溶性蛋白质聚集体的表征为揭示蛋白质聚集过程的机制以及评价蛋白质治疗药物的活性和安全性提供了有价值的信息。然而，非共价相互作用，瞬时性质和较高程度的结构异质性的可溶性聚集系统阻碍了精确的表征在分子水平上。在这里，我们描述的方法，使用本地质谱加上温控电喷雾电离和尺寸排阻色谱监测的聚集过程和配置文件的聚集体详细。
Characterization of soluble protein aggregates provides valuable information for revealing mechanisms of protein aggregation process and assessing the activity and safety of protein therapeutics. However, the noncovalent interaction, the transient nature and higher degree of structural heterogeneity of the soluble aggregation system hinders precise characterization at the molecular level. Here, we describe methods using native mass spectrometry coupled with temperature-control electrospray ionization and size-exclusion chromatography to monitor the aggregation process and profile the aggregates in detail.