A New Soluble Gelatin Sponge for Transcatheter Hepatic Arterial Embolization
A New Soluble Gelatin Sponge for Transcatheter Hepatic Arterial Embolization
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DOI：
10.1007/s00270-010-9866-2
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发表时间：
2010-12-01
影响因子：
2.9
通讯作者：
Sonomura, Tetsuo
中科院分区：
文献类型：
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作者：
Takasaka, Isao;Kawai, Nobuyuki;Sonomura, Tetsuo
为了制备可溶性明胶海绵（GS）并探索当进行经导管肝动脉栓塞时抑制侧支通路发展的GS颗粒（GSPs），通过50和100 kDa的再生医学明胶（RM-G）获得了我们机构的研究动物护理机构委员会的批准，通过冷冻干燥并加热至110-150 ° C的温度以进行交联来制备RM-G海绵。体外测量GSPs 8只猪经导管肝动脉栓塞分为两组，6只接受135 ℃/50 RM-GSPs，125 ℃/100 RM-GSPs和138 ℃/50 RM-GSPs，体外溶解时间为48小时或更长，两只猪接受GelpartGSPs（G-GSPs），不溶时间为14天，作为对照。在每只猪的肝动脉的两个分支上进行经动脉化疗栓塞，在110-138 ℃的温度下加热的RM-GSPs是可溶的。GSPs随温度升高而增加，用G-GSP栓塞的肝支在6天后仍然闭塞，用135 ℃/50 RM-GSP和125 ℃/100 RM-GSP栓塞肝支，3天后观察到侧支循环的发展，并在4小时内保持闭塞，1天后观察到再通。GS保持闭塞1天，2天后观察到再通，没有侧支通路的发展。在通过调节加热温度制备的具有不同溶解时间的RM-GS中，138 ℃/50 RM-GSP是具有最长闭塞时间而不诱导侧支通路发展的可溶性GSP。
To prepare a soluble gelatin sponge (GS) and to explore the GS particles (GSPs) that inhibit development of collateral pathways when transcatheter hepatic arterial embolization is performed The approval of the Institutional Committee on Research Animal Care of our institution was obtained By means of 50 and 100 kDa of regenerative medicine gelatin (RM-G), RM-G sponges were prepared by freeze-drying and heating to temperatures of 110-150 degrees C for cross-linkage The soluble times of RM-GSPs were measured in vitro Eight swine for transcatheter hepatic arterial embolization were assigned into two groups six received 135 degrees C/50RM-GSPs, 125 degrees C/100RM-GSPs and 138 degrees C/50RM-GSPs, with soluble time of 48 h or more in vitro, two swine received Gelpart GSPs (G-GSPs) with insoluble time of 14 days as a control Transartenal chemoembolization was performed on two branches of the hepatic artery per swine RM-GSPs heated at temperatures of 110-138 degrees C were soluble Mean soluble times of the RM-GSPs increased with higher temperature Hepatic branches embolized with G-GSP remained occluded after 6 days, and development of collateral pathways was observed after 3 days Hepatic branches embolized with 135 degrees C/50RM-GSP and 125 degrees C/100RM-GSP remained occluded for 4 h and recanalization was observed after 1 day Hepatic branches embolized with 138 degrees C/50RM-GS remained occluded for I day, and recanalization was observed after 2 days with no development of collateral pathways In RM-GSs with various soluble times that were prepared by modulating the heating temperature, 138 degrees C/50RM-GSP was the soluble GSP with the longest occlusion time without inducing development of collateral pathways.