High-capacity thermo-responsive magnetic molecularly imprinted polymers for selective extraction of curcuminoids
High-capacity thermo-responsive magnetic molecularly imprinted polymers for selective extraction of curcuminoids
复制标题
用于选择性提取类姜黄素的高容量热响应磁性分子印迹聚合物
DOI：
10.1016/j.chroma.2014.05.040
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发表时间：
2014-08-08
影响因子：
4.1
通讯作者：
Chen, Xiaoqin
中科院分区：
文献类型：
--
作者：
You, Qingping;Zhang, Yuping;Chen, Xiaoqin
首次开发出具有高容量和选择性的用于选择性识别姜黄素的热响应磁性分子印迹聚合物（TMMIP）。通过 TEM、FT-IR、TGA、VSM 和 UV 对所得 TMMIP 进行了表征，表明 TMMIP 表现出热响应性 [较低临界溶液温度 (LCST) 为 33.71 ℃] 和快速磁分离 (5 s)。对聚合、吸附和释放条件进行了详细优化，以获得最高的结合容量、选择性和释放比。我们发现，所采用的热响应单体[N-异丙基丙烯酰胺（NIPAm）]不仅可以被视为具有热响应性的惰性聚合物主链，而且可以被视为与碱性单体（4-VP）组合的功能性共聚单体，当在结合溶液中添加乙醇时，可以获得更特异的结合位点。在45℃（LCST以上）的20%（v/v）乙醇溶液中，收缩TMMIPs上姜黄素选择性最高的最大吸附容量为440.3μg/g（无热敏性MMIPs的1.93倍），在20℃（LCST以下）的甲醇乙酸（9/1，v/v）溶液中最大释放比例约为98%。膨胀的TMMIP。姜黄素和TMMIPs之间的吸附过程遵循Langumuir吸附等温线和准一级反应动力学。制备的 TMMIP 还表现出高重现性（RSD
Thermo-responsive magnetic molecularly imprinted polymers (TMMIPs) for selective recognition of curcuminoids with high capacity and selectivity have firstly been developed. The resulting TMMIPs were characterized by TEM, FT-IR, TGA, VSM and UV, which indicated that TMMIPs showed thermo-responsiveness [lower critical solution temperature (LCST) at 33.71 degrees C] and rapid magnetic separation (5 s). The polymerization, adsorption and release conditions were optimized in detail to obtain the highest binding capacity, selectivity and release ratio. We found that the adopted thermo-responsive monomer [N-isopropylacrylamide (NIPAm)] could be considered not only as inert polymer backbone for thermo-responsiveness but also as functional co-monomers combination with basic monomer (4-VP) for more specific binding sites when ethanol was added in binding solution. The maximum adsorption capacity with highest selectivity of curcumin was 440.3 mu g/g (1.93 times that on MMIPs with no thermosensitivity) at 45 degrees C (above LCST) in 20% (v/v) ethanol solution on shrunk TMMIPs, and the maximum release proportion was about 98% at 20 degrees C (below LCST) in methanol acetic acid (9/1, v/v) solution on swelled TMMIPs. The adsorption process between curcumin and TMMIPs followed Langumuir adsorption isotherm and pseudo-first-order reaction kinetics. The prepared TMMIPs also showed high reproducibility (RSD