CC chemokine receptor 4 ligand production by bronchoalveolar lavage fluid cells in cigarette-smoke-associated acute eosinophilic pneumonia
CC chemokine receptor 4 ligand production by bronchoalveolar lavage fluid cells in cigarette-smoke-associated acute eosinophilic pneumonia
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DOI：
10.1016/j.clim.2005.03.001
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发表时间：
2005-07-01
影响因子：
8.6
通讯作者：
Tsuda, T
中科院分区：
文献类型：
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作者：
Nureki, S;Miyazaki, E;Tsuda, T
我们检测了烟雾相关性急性嗜酸性粒细胞性肺炎（CS-AEP）患者支气管肺泡灌洗液（BALF）细胞产生的巨噬细胞源性趋化因子（MDC/CCL 22）和胸腺活化调节趋化因子（TARC/CCL 17）。CS-AEP患者BALF中CC趋化因子受体4（CCR 4）配体水平显著高于健康志愿者，并与Th 2细胞因子水平相关。白细胞介素-4增强CCR 4配体的产生。在CS-AEP患者和健康对照吸烟者的CD 68阳性细胞中观察到MDC表达。相比之下，仅在CS-AEP中检测到CD 68或CD 1a阳性细胞中的TARC表达。体内香烟烟雾激发试验诱导BALF和BALF贴壁细胞培养上清液中CCR 4配体增加。这些结果表明，肺泡巨噬细胞和树突状细胞通过产生CCR 4配体参与CS-AEP的发病机制，可能是响应于香烟烟雾。(c)2005年爱思唯尔公司All rights reserved.
We examined the production of macrophage-derived chemokine (MDC/CCL22) and thymus- and activation-regulated chemokine (TARC/CCL17) by bronchoalveolar lavage fluid (BALF) cells in cigarette-smoke-associated acute eosinophilic pneumonia (CS-AEP). The CC Chemokine Receptor 4 (CCR4) ligand levels in BALF from patients with CS-AEP were considerably higher than those in healthy volunteers and correlated well with Th2 cytokine levels. Interleukin-4 enhanced CCR4 ligand production. MDC expression was observed in CD68-positive cells from patients with CS-AEP and in healthy control smokers. In contrast, TARC expression in CD68- or CD1a-positive cells was detected only in CS-AEP. An in vivo cigarette smoke challenge test induced increases in CCR4 ligands in the BALF and in the cultured supernatant of BALF adherent cells. These results suggest that alveolar macrophages and dendritic cells contribute to the pathogenesis of CS-AEP by generating CCR4 ligands, probably in response to cigarette smoke. (c) 2005 Elsevier Inc. All rights reserved.