Interaction of a tyrosine kinase from human sperm with the zona pellucida at fertilization.
Interaction of a tyrosine kinase from human sperm with the zona pellucida at fertilization.
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受精时人类精子的酪氨酸激酶与透明带的相互作用。
DOI：
10.1126/science.7541556
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Saling,PM
中科院分区：
文献类型：
--
作者：
Burks,DJ;Carballada,R;Moore,HD;Saling,PM
具有蛋白酪氨酸激酶特征的95千道尔顿小鼠精子蛋白已被鉴定为ZP 3的受体，ZP 3是卵子细胞外基质中的糖蛋白。通过筛选来自人睾丸的表达文库来确定人类同源物的结构;分离出睾丸特异性互补DNA，其编码类似于受体酪氨酸激酶的蛋白质，并且似乎仅在睾丸生殖细胞中表达。针对来自细胞内结构域的合成肽的抗体识别含有磷酸酪氨酸的95千道尔顿的人精子蛋白;人ZP 3刺激该精子蛋白的激酶活性。合成肽对应于预测的细胞外结构域的区域抑制精子结合到人透明质酸。ZP 3受体一级序列的可用性为精子靶向避孕药的开发提供了一个合理的起点。
A 95-kilodalton mouse sperm protein with characteristics of a protein tyrosine kinase has been identified as a receptor for ZP3, a glycoprotein in the egg's extracellular matrix. The structure of the human homolog was determined by screening an expression library from human testis; a testis-specific complementary DNA was isolated that encodes a protein similar to receptor tyrosine kinases and appears to be expressed only in testicular germ cells. Antibodies against a synthetic peptide from the intracellular domain recognized a 95-kilodalton human sperm protein that contains phosphotyrosine; human ZP3 stimulates the kinase activity of this sperm protein. Synthetic peptides corresponding to regions of the predicted extracellular domain inhibited sperm binding to human zona pellucida. Availability of the primary sequence of a receptor for ZP3 provides a rational starting point for sperm-targeted contraceptive development.